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高久翔,张宇纯,于 亮.电针对小鼠下肢肌萎缩干预效果的研究[J].中国康复医学杂志,2018,(3):280~285
电针对小鼠下肢肌萎缩干预效果的研究    点此下载全文
高久翔  张宇纯  于 亮
北京体育大学运动人体科学学院,北京,100084
基金项目:国家体育总局全民健身课题(2015B046);北京体育大学国家级教学示范中心资助课题
DOI:
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摘要:
      摘要 目的:为了研究中频率电针刺激对小鼠比目鱼肌肌萎缩恢复的影响。 方法:60只C57BL/6小鼠,其中30只随机分为空白对照组(Con)和萎缩造模组(Atro),1周后验证此模型,将另30只造模致其肌萎缩后随机分为自然恢复组(Rec)、运动干预组(Exer)、电针干预组(Elec)。Atro组对小鼠进行后肢石膏固定1周;Rec组在拆下石膏后自由饲养2周;Exer组采取跑台运动干预2周;Elec组对小鼠后肢进行电针刺激2周。冰冻切片免疫荧光检测肌纤维横截面积、ATPase染色检测肌纤维类型变化、Western Blot检测Troponin I-SS(TNNI1)、Troponin I-FS(TNNI2)蛋白表达。 结果:Atro组肌湿重和肌纤维横截面积明显低于Con组(P<0.01),TNNI1、TNNI2蛋白表达与Con组相比同样具有显著性差异(P<0.05)。肌萎缩后Exer组、Elec组的慢肌、快肌的横截面积与Rec组相比均显著增加(P<0.05)。TNNI1蛋白表达量都有非常显著性升高(P<0.01),TNNI2蛋白无明显差异(P>0.05)。 结论:中频率电针干预可有效增加肌纤维横截面积,尤其增加慢肌纤维TNNI1和TNNI2蛋白的表达,与运动干预一样都是一种促使废用性肌萎缩康复的有效方法。
关键词:废用性肌萎缩  骨骼肌纤维类型  肌钙蛋白  电针
Electric acupuncture intervention on the effects of disuse muscular atrophy muscle fiber types of mice    Download Fulltext
Sport Science College, Beijing Sport University, Beijing,100084
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Abstract:
      Abstract Objective: To study the frequency of electrical stimulation affect the atrophy recovery of mouse soleus muscle. Method: Sixty mice, 30 of them were randomly divided into blank control group (Con) and atrophy model group (Atro) and validated this model after 1 week. The other 30 mice were randomly divided into three groups: the natural recovery group (Rec), the exercise intervention group (Exer), the electroacupuncture intervention group (Elec) after making them atrophy. Atrophy model group were plaster fixed one week. Natural recovery group was natural fed two weeks after removing the plaster. Exercise intervention group runs 1 hour at 16m/min speed two weeks. EA intervention group were stimulated 15min daily for 2 weeks on hind limb, with pulse duration 0.35ms, frequency 50Hz, for 2 weeks. Frozen sections and Immunofluorescence were used to measure the cross-sectional area of muscle fibers. ATPase staining was used to detect the muscle fiber type changing, and Western Blot to detect soleus muscle Troponin I-SS (TNNI1) and Troponin I-FS (TNNI2) protein expression. Result: The soleus muscle in Atro group showed significant change in cross-sectional area and wet weight compared with Con group(P<0.01), The expression of TNNI1 and TNNI2 protein was also significantly different from that of Con group (P<0.05). Compared with the Rec group, the cross-sectional area of slow muscle and fast muscle in the Exer and Elec groups significantly increased (P<0.05), TNNI1 protein expression significantly increased (P<0.01), TNNI2 protein was no significant difference detected(P>0.05). Conclusion: The middle low frequency electroacupuncture intervention can effectively increase the cross-sectional area of muscle fibers, in particular, increase the TNNI1 and TNNI2 protein expression of slow muscle fibers. It is as effective as exercise intervention to promote recovery of disuse muscular atrophy.
Keywords:disuse muscle atrophy  skeletal muscle fiber types  troponin  electroacupuncture
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