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何源源,徐锦江.有氧运动对乳腺癌大鼠癌因性疲乏后骨骼肌中线粒体动力学与功能的影响[J].中国康复医学杂志,2018,(8):885~890
有氧运动对乳腺癌大鼠癌因性疲乏后骨骼肌中线粒体动力学与功能的影响    点此下载全文
何源源  徐锦江
锦州医科大学,锦州,121001
基金项目:辽宁省科技厅课题(2013225305)
DOI:
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摘要:
      摘要 目的:探讨乳腺癌大鼠癌因性疲乏后骨骼肌中线粒体动力学和功能变化及有氧运动干预对其的影响。 方法:将24只雌性SD大鼠随机分为正常组、模型组和运动组,每组8只。除正常组外,其余各组均皮下注射鼠乳腺癌SHZ-88细胞株并接受化疗,以建立乳腺癌化疗疲乏动物模型;用水迷宫实验和力竭游泳实验检测各组大鼠癌因性疲乏状态;运动组鼠分别接受20min的中等强度的游泳运动,1次/天,6天/周,共6周。6周后检测大鼠癌因性疲乏情况,血浆中三磷酸腺苷(ATP)含量,骨骼肌中线粒体分裂蛋白(Drp1)和融合蛋白(Mfn1)的表达情况,线粒体膜电位(MMP),线粒体内ATP、丙二醛(MDA)和谷胱甘肽(GSH)的含量。 结果:①化疗后,与正常组比较,模型组与运动组均出现疲乏(P<0.05);与正常组比较,模型组血浆中ATP水平降低,骨骼肌中Drp1表达增加,Mfn1表达降低,MMP降低,MDA合成增多和GSH合成减少(P<0.05)。②运动后,与模型组相比,运动组疲乏状态明显好转,血浆ATP水平升高,骨骼肌中Drp1表达减少,Mfn1表达增多,MMP升高,MDA合成减少和GSH合成增多(P<0.05)。 结论:中等强度有氧运动可以明显缓解乳腺癌大鼠的癌因性疲乏,可能与调节骨骼肌中线粒体动力学、改善线粒体功能有关。
关键词:有氧运动  乳腺癌  癌因性疲乏  线粒体动力学  线粒体功能
Effects of aerobic exercise on the function and dynamics of mitochondrial in rats’ skeletal muscle after breast cancer-related fatigue    Download Fulltext
Jinzhou Medical University, Jinzhou, 121001
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Abstract:
      Abstract Objective: To study the changes of function and dynamics of mitochondrial in breast cancer-related fatigue rats and the effects of aerobic exercise on these changes. Method: Twenty-four female SD rats were randomly divided into normal group, model group and exercise group, 8 rats in every group. Except the normal group, the rats of other groups were injected with the SHZ-88 cell line and received chemotherapy to establish the model of breast cancer-related fatigue. Water maze and swimming capability experiments were conducted to determine the cancer-related fatigue of the animal models. Exercise group received swimming exercise for 20mins, daily, 6d/week, lasting for 6 weeks. After 6 weeks training, the cancer-related fatigue and dynamin-related protein 1 (Drp-1) as well as fusion protein (Mfn1) in the mitochondrial of the skeletal were measured. The levels of adenosine triphosphate (ATP) in the plasma, membrane potential (MMP), malondialdehyde (MDA), reduced glutathione (GSH) in the mitochondrial of the skeletal were detected. Result: ①Compared to the normal group,the model group and exercise group suffered from fatigue after chemotherapy (P<0.05). Compared to the normal group, the level of ATP in the plasma was higher in model group;the protein expression level of Drp1 was remarkably increased; the protein expression of Mfn1 was significantly decreased; The level of MMP was lower; The level of MDA increased, the level of GSH decreased(P<0.05). ②After exercise, compared to model group, the cancer-related fatigue in exercise groups obviously relieved(P<0.05); The level of ATP in the plasma was higher; The protein expression level of Drp1 was remarkably decreased, The protein expression of Mfn1 was significantly increased; The level of MMP was higher; the levels of MDA decreased, the levels of GSH increased (P<0.05). Conclusion: The moderate intensity aerobic exercises can reduce rats' cancer-related fatigue significantly, which may be related to the regulation of the mitochondrial dynamics and improvement of the mitochondrial function.
Keywords:aerobic exercise  breast cancer  cancer-related fatigue  mitochondrial dynamics  mitochondrial function
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