| 万峪岑,孙 师,冯智萍,赵利娜,冯思宁,尹艳梅,周禹鑫,张志强,张立新.小剂量超短波治疗对大鼠脊髓损伤后VEGF和BDNF的影响[J].中国康复医学杂志,2018,(8):891~895 |
| 小剂量超短波治疗对大鼠脊髓损伤后VEGF和BDNF的影响 点此下载全文 |
| 万峪岑 孙 师 冯智萍 赵利娜 冯思宁 尹艳梅 周禹鑫 张志强 张立新 |
| 中国医科大学附属盛京医院康复中心，辽宁沈阳，110022 |
| 基金项目：国家自然科学基金青年基金资助项目（81101462）； 辽宁省自然科学基金项目（201602059）；辽宁省科学事业公益研究基金项目（2016003001） |
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| 摘要
目的：观察小剂量超短波治疗对大鼠脊髓损伤后不同时间点的功能恢复、血管内皮生长因子（VEGF）及脑源性神经营养因子（BDNF）表达的影响,并探讨其作用机制。
方法：54只SD大鼠，被随机分成3组：假手术组，暴露硬脊膜后，不予打击，直接缝合；对照组，采用Allen打击法建立大鼠脊髓损伤（spinal cord injury,SCI）模型，不给予任何治疗；超短波组，在SCI后第2天开始给予受损部位小剂量超短波治疗，每日1次，每次7min，治疗至取材前。于造模后第2天，1周、2周、3周、4周采用BBB评分评定大鼠的后肢运动功能，免疫组织化学染色检测VEGF、BDNF的表达，应用图像分析系统进行半定量分析。
结果：BBB评分显示随着损伤时间的延长，脊髓损伤各组大鼠运动功能逐渐改善，与对照组相比，超短波组自术后1周起运动功能明显改善（P<0.01)。免疫组化染色结果显示术后1周起，对照组、超短波组的VEGF、BDNF阳性表达较假手术组明显增加（P<0.001)，2周时两组的VEGF、BDNF表达均达高峰后逐渐下降，超短波组高峰持续时间均延长。4个时间点超短波组的VEGF表达较对照组明显增加（P<0.01,P<0.05,P<0.01,P<0.001）；1周、4周超短波组的BDNF表达较对照组明显增加（P<0.01,P<0.05）。
结论：脊髓损伤后超短波治疗可以增加VEGF的表达，改善组织血液循环，延长BDNF蛋白高峰持续时间，促进神经功能恢复。 |
| 关键词：脊髓损伤 超短波 血管内皮生长因子 脑源性神经营养因子 |
| Effects of low dose ultrashort wave therapy on expression of VEGF and BDNF following spinal cord injury in rats Download Fulltext |
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| Shengjing Hospital of China Medical University, Shenyang,Liaoning,110022 |
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| Abstract: |
| Abstract
Objective: To investigate the effect of ultrashort wave therapy on the function recovery,the expression of vascular endothelial growth factor (VEGF) and brain derived nerve neurotrophic factor (BDNF) after spinal cord injury (SCI) in rats.
Method:Female SD rats（n=54） were randomly divided into three groups,sham-operated, control group，that were subjected to spinal cord contusion without any treatment,ultrashort wave group, SCI animals exposed to USW radiation 7min at 24h after injury, once per day until the animals were sacrificed. Locomotors functional recovery was assessed 1d and every week post operation period by performing open-field locomotors tests in BBB score,Immunohistochemical staining was performed to observe the expression of the VEGF and BDNF.The image analysis system was used for semi quantitative analysis.
Result:From 1 week after operation, BBB scores of ultrashort wave group were higher than control group（P<0.01), Immunohistochemical staining showed that 1w post operation,VEGF and BDNF positive expression of control group and ultrashort wave group compared with the sham-operated group was significantly increased (P<0.001), the expression of VEGF and BDNF in the two groups reached the peak at 2w and were gradually decreased, the peak durations of ultrashort wave group was prolonged. Compared with the control group, VEGF positive expression of ultrashort wave group were significantly increased at each time point（P<0.01,P<0.05,P<0.01,P<0.001）, BDNF positive expression of ultrashort wave group were significantly increased at 1w and 4w（P<0.01,P<0.05）.
Conclusion:Ultrashort wave therapy can increase the expression of VEGF after SCI, improve the blood circulation of organization, prolong the peak duration of BDNF, promote the recovery of neurological function. |
| Keywords:spinal cord injury ultrashort wave vascular endothelial growth factor brain-derived neurotrophic factor |
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