| 黄煦格,何剑全,陈 健,王 潇,黄 慧.脉冲电磁场对卵巢切除大鼠骨组织活化T细胞核因子2和空泡型V-ATP酶mRNA表达的影响[J].中国康复医学杂志,2018,(8):896~900 |
| 脉冲电磁场对卵巢切除大鼠骨组织活化T细胞核因子2和空泡型V-ATP酶mRNA表达的影响 点此下载全文 |
| 黄煦格 何剑全 陈 健 王 潇 黄 慧 |
| 厦门大学附属中山医院康复医学科，厦门，361004 |
| 基金项目：国家自然科学基金（81272168）；福建省自然科学基金（2016J01623，2017J01375）；福建省卫生计生委青年科研课题（2015-2-45） |
| DOI： |
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| 摘要： |
| 摘要
目的：探讨脉冲电磁场（pulsed electromagnetic fileds, PEMF）对卵巢切除(ovariectomized，OVX)大鼠骨组织细胞核因子κB 受体活化因子(receptor activator of NF-κB, RANK)，活化T细胞核因子2(nuclear factor of activated T2, NFAT2)和空泡型V-ATP酶（vavcuolar H+-ATPase, V-ATP）表达的影响。
方法：将48只SD大鼠随机分为假手术组(SHAM)，卵巢切除组(OVX)，卵巢切除+脉冲电磁场治疗组（OVX+PEMF）。OVX+PEMF组大鼠在频率8Hz，磁场强度3.8mT的PEMF下每天干预40min，干预8周和16周后检测大鼠骨密度和骨组织RANK，NFAT2和V-ATP的mRNA表达水平。
结果：OVX组BMD在第8周（P<0.05）和第16周（P<0.001）均低于SHAM组。SHAM组与OVX组比较，8周时RANK表达P>0.05，而SHAM组NFAT2与V-ATP表达均低于OVX组（P<0.05，P<0.01）；16周时RANK、NFAT2、V-ATP在OVX组表达升高（均P<0.01）。SHAM组与OVX+PEMF组比较，8周时两组RANK、NFAT2、V-ATP表达P>0.05；16周时OVX+PEMF组RANK和NFAT2表达均高于SHAM组（均P<0.01），而两组V-ATP表达P>0.05。OVX组与OVX+PEMF组相比，8周时两组RANK和V-ATP表达均P>0.05，OVX+PEMF组NFAT2表达低于OVX组（P<0.05）；第16周时两组RANK表达P>0.05，NFAT2和V-ATP在OVX+PEMF组的表达均下降（P<0.01，P<0.05）。
结论：PEMF可通过下调OVX大鼠骨组织中NFAT2和V-ATPmRNA的表达从而抑制破骨细胞的骨吸收，进而延缓OVX大鼠的骨丢失。 |
| 关键词：脉冲电磁场，细胞核因子κB 受体活化因子 活化T细胞核因子2 空泡型V-ATP |
| Effects of pulsed electromagnetic fields on the expression of NFAT2 and V-ATP mRNA in the bone of ovariectomized rats Download Fulltext |
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| Department of Rehabilitation, Zhongshan Hospital Xiamen University, Xiamen Fujian, 361004, P.R.China. |
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| Abstract: |
| Abstract
Objective: To investigate the effect of Pulsed electromagnetic fields (PEMF) on the mRNA expression of Receptor activator of NF-κB (RANK), Nuclear factor of activated T2 (NFAT2) and Vacuolar H+-ATPase(V-ATP) in the bone of ovariectomized(OVX) rats.
Method: Forty eight Sprague-Dawley rats were randomly divided into SHAM group, OVX group and OVX+PEMF group. The rats in OVX+PEMF group were treated in 8Hz and 3.6 mT PEMF for 40 minutes once a day. The level of Bone mineral density (BMD) was detected after intervention. Moreover, the mRNA expression of RANK, NFAT2 and V-ATP in bone were tested in every group at 8th and 16th weeks respectively.
Result: The BMD of OVX group was lower than that of SHAM group at the 8th week (P<0.05) and the 16th week (P<0.001). Comparison between SHAM group and OVX group at the 8th week, there was no significant difference in the expression of RANK mRNA (P>0.05), while the expression of NFAT2 and V-ATP were increased in OVX group (P<0.05, P<0.01). Furthermore, the expressions of RANK, NFAT2 and V-ATP in OVX group were higher than those in SHAM group at the 16th week (all P<0.01). The mRNA expressions of RANK, NFAT2 and V-ATP had no significant changes between SHAM group and OVX+PEMF group at the 8th week (P>0.05). At the 16th week, the expressions of RANK and NFAT2 in OVX+PEMF group were higher than those in SHAM group (all P<0.01), but no significant difference for the expression of V-ATP (P>0.05). Compared to OVX group and OVX+PEMF group, the mRNA expressions of RANK and V-ATP at 8th weeks had no difference (P>0.05). However, the expression of NFAT2 was down-regulated in OVX+PEMF group (P<0.05). The RANK expression of the two groups had no change at the 16th week (P>0.05), while the expressions of NFAT2 and V-ATP were decreased in OVX+PEMF group (P<0.01, P<0.05).
Conclusion: PEMF can reduce the bone resorption of osteoclasts by down-regulating the mRNA expressions of NFAT2 and V-ATP, and then delay bone loss in OVX rats. |
| Keywords:pulsed electromagnetic fields, RANK, NFAT2, V-ATP |
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