| 梅紧紧,李婧雯,王艺莹,张建云,张丽红,郭 健,李瑞青.督脉电针对脑卒中后肢体痉挛大鼠大脑皮质中KCC2、GABAARα1表达的影响[J].中国康复医学杂志,2025,(4):487~494 |
| 督脉电针对脑卒中后肢体痉挛大鼠大脑皮质中KCC2、GABAARα1表达的影响 点此下载全文 |
| 梅紧紧 李婧雯 王艺莹 张建云 张丽红 郭 健 李瑞青 |
| 河南中医药大学，河南省郑州市，450000 |
| 基金项目：河南省高等学校重点科研项目（21A360023）；河南省中医药科学研究专项（2019ZY2129）；国家青年自然科学基金项目（81503630） |
| DOI：10.3969/j.issn.1001-1242.2025.04.002 |
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| 摘要： |
| 摘要
目的：观察督脉电针对脑卒中后肢体痉挛大鼠大脑皮质中钾离子-氯离子共转运体2 （KCC2）和 γ-氨基丁酸A型受体（GABAARα1）表达的影响，探讨其治疗脑卒中后肢体痉挛的可能机制。
方法：选用45只SPF级雄性SD大鼠，随机选出空白组和假手术组，每组各9只，剩余大鼠制备大脑中动脉栓塞模型。在完成造模后的第3天，经一系列行为学检测后，筛选出肢体痉挛的大鼠，并随机分为模型组、电针组和巴氯芬组，每组各9只。电针组给予针刺“大椎”“脊中”“后会”等穴位，巴氯芬组给予灌胃巴氯芬溶液，均连续治疗7d。以神经功能缺损评分观察各组大鼠神经功能变化，以改良Ashworth分级评分和肌电信号值的改变评估大鼠肌张力水平变化，用实时荧光PCR法和Western Blot法检测大鼠大脑皮质中KCC2和GABAARα1 mRNA和蛋白的表达情况；用比色试剂盒检测氯离子含量改变情况。
结果：与同时点假手术组比较，模型组神经功能缺损评分、改良Ashworth分级评分、脑组织含水量、脑梗死体积及氯离子含量均升高（P<0.01），肌电信号值、大脑皮质KCC2和GABAARα1 mRNA、蛋白表达量均降低（P<0.01）；与模型组比较，电针组和巴氯芬组神经功能缺损评分、改良Ashworth分级评分、脑组织含水量、脑梗死体积及氯离子均降低（P<0.01），肌电信号值及大脑皮质KCC2和GABAARα1 mRNA、蛋白表达均升高（P<0.01，P<0.05）；而督脉电针组与巴氯芬组相较，各项指标无明显差异（P>0.05）。
结论：督脉电针可升高大鼠大脑皮质KCC2、GABAARα1 mRNA及其蛋白的表达，发挥γ-氨基丁酸的抑制作用，进而改善脑卒中后肢体痉挛症状。 |
| 关键词：督脉电针 脑卒中肢体痉挛 钾离子-氯离子共转运体2 γ-氨基丁酸A受体 氯离子含量 |
| Effects of governor vessel electroacupuncture on the expression of KCC2 and GABAARα1 in cerebral cortex of rats with post-stroke limb spasticity Download Fulltext |
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| Henan University of Chinese Medicine， Zhengzhou， Henan， 450000 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To observe the effect of governor vessel （GV） electroacupuncture on potassium chloride cotransporter 2 （KCC2） and γ- Aminobutyric acid type a receptor （GABAAR α 1）and to explore the possible mechanism of GV electroacupuncture in improving post-stroke limb spasticity （PSLS）.
Method： 45 male SD rats of SPF grade were randomly selected into the blank group and the sham operation group, with 9 rats in each group. The remaining rats were used to make the middle cerebral artery occlusion model. On the 3rd day after the completion of modeling, the rats with limb spasticity were chosen and randomly divided into the model group, the electroacupuncture group, and the baclofen group, with 9 rats in each group, following a series of behavioral tests. Acupuncture was provided to the electroacupuncture group at "Dazhui", "Jizhong" and "Houhui", whereas the baclofen group received a baclofen solution by gavage for 7 days.Rats in each group were observed for the changes in nerve function by the symptom score of nerve function defect, and the changes in muscle tension level were evaluated by the modified Ashworth grading score and EMG signal value change. KCC2 and GABAARα1 mRNA and protein in the cerebral cortex of rats were detected by qPCR and Western Blot, and changes in chloride ion content were detected with a colorimetric kit.
Result: The neurological function defect score, the modified Ashworth grading score， the water content of brain tissue, the volume of cerebral infarction, and the expression of chloride ion content in the model group were all significantly increased compared with the sham operation group at the same time point (P<0.01), while the EMG signal value, cerebral cortex KCC2 and GABAAR α 1 mRNA and protein were significantly decreased (P<0.01). Compared with the model group, the nerve function defect score， the modified Ashworth grading score, the water content of brain tissue, the volume of cerebral infarction and the expression of chloride ion content in the electroacupuncture group and the baclofen group were all decreased （P<0.01）， while the EMG signal value and KCC2, with GABAARα1 mRNA and protein expression were all increased (P<0.01, P<0.05, respectively）； There was no significant difference in all indexes between the GV electroacupuncture group and the baclofen group （P>0.05）.
Conclusion: GV electroacupuncture can increase the expression of KCC2， GABAARα1 mRNA,and protein in the cerebral cortex of rats，in order to exert the inhibitory effect of γ-aminobutyric acid，thus improving the symptoms of PSLS. |
| Keywords:governor vessel electroacupuncture post-stroke limb spasticity KCC2 GABAARα1 chloride ion content |
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