| 李洪亮,向 娟,欧阳里知,陈 果,薛智慧,龙抗胜,李铁浪.电针对大脑中动脉梗塞大鼠EphrinB2/EphB2信号通路上突触重塑相关因子的影响[J].中国康复医学杂志,2016,(10):1067~1077 |
| 电针对大脑中动脉梗塞大鼠EphrinB2/EphB2信号通路上突触重塑相关因子的影响 点此下载全文 |
| 李洪亮 向 娟 欧阳里知 陈 果 薛智慧 龙抗胜 李铁浪 |
| 湖南中医药大学第一附属医院，湖南长沙，410007 |
| 基金项目：湖南省高校创新平台开放基金项目(14K070)；湖南省中管局重点项目（201310）；湖南省研究生创新基金项目(CX2014B360) |
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| 摘要
目的：观察电针对大脑中动脉梗塞模型(MCAO)大鼠促红细胞生成素产生肝细胞配体-B2（erythropoietin-producing hepatocyte receptor interacting protein-B2, EphrinB2）/受体-B2（erythropoietin-producing hepatocyte receptor-B2, EphB2）信号通路上突触重塑相关因子表达的影响，以期揭示针刺治疗脑缺血的可能机制。
方法：120只雄性SD大鼠随机分为假手术组、模型组、穴位组、非穴位组（n=30）；各组再分为术后3d，14d及21d三个亚组（n=10）。除假手术组外，其余各组大鼠用Zea-longa线栓法制备MCAO模型；穴位组和非穴组电针治疗，其余两组只绑不针。对大鼠行神经功能评分，采用免疫组化、免疫荧光和蛋白质印迹法行相应指标检测。
结果：模型组术后3d梗死灶周EphrinB2/EphB2表达明显减少，但随时间呈递增趋势，21d恢复到假手术水平。穴位组较模型组和非穴组增加更明显，术后21d表达高于假手术组（P<0.05）。Ras相关的C3肉毒毒素底物1（ras-related C3 botulinum toxin substrate-1，Rac-1）和微管相关蛋白-2（microtubule-associated protein-2，MAP-2）的表达随时间逐渐递增，且均与神经功能改善相吻合；其中穴位组增加最明显，术后21d超假手术组水平（P<0.05）。
结论：EphrinB2/EphB2信号通路参与了MCAO大鼠突触重塑，电针肝俞、肾俞穴可能通过激活EphrinB2/EphB2信号通路，促进MCAO大鼠神经功能的康复。 |
| 关键词：脑缺血 EphrinB2/EphB2信号通路 肝俞穴、肾俞穴 电针 |
| Effects of electroacupuncture on the synaptic plasticity related factors of EphrinB2/EphB2 pathway in MCAO rats Download Fulltext |
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| The First Affliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha City,Hunan Province,410007 |
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| Abstract: |
| Abstract
Objective: To observe the effect of electroacupuncture on EphrinB2 (Erythropoietin-producing hepatocyte receptor interacting protein-B2)/EphB2 (Erythropoietin-producing hepatocyte receptor-B2, EphB2) pathway and its synaptic plasticity related factors in MCAO rats.
Method: A total of 120 healthy male SD rats were randomly and equally divided into four groups: the sham-operated group, the model group, the acupuncture group and the nonacupuncture point group. After that, each group was divided into three subgroups: 3d, 14d and 21d postoperation. Samples were duplicated into models of MACO with thread-occlusion method. Nerve functional scoring was evaluated and at the same time, immunohistochemistry and western blot were adopted to detect the EphrinB2/EphB2, Rac-1(Ras-related C3 botulinum toxin substrate-1) and MAP-2(Microtubule-associated protein-2) expression in MCAO rats.
Result: The expression of EphB2/EphrinB2 in the infarct periphery was decreased after MACO and then gradually increased with the time. The increased expression of EphB2/EphrinB2 of the acupuncture group were dramatically when compared with the model group and the nonacupuncture point group (P<0.05) and exceeded the level of the sham group on 21d after operation(P<0.05). The expression of Rac-1 and MAP-2 were gradually increased with the time and reached the level of the sham group on 21d after operation. The expression of Rac-1 and MAP-2 were significantly increased in the model group comparing with the nonacupoint group and exceeded the level of the sham group on 21d after operation (P<0.05).
Conclusion: The electroacupuncture treatment on Ganshu, Shenshu points can improve the neurologic function of MCAO rats, which may due to the gradually enhanced of EphrinB2/EphB2 pathway and its synaptic plasticity related factors,and the upregulation of synaptic plasticity in the infarct periphery. |
| Keywords:cerebral ischemia EphB2/EphrinB2 pathway Ganshu(BL18) Shenshu(BL23) electroacupuncture |
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