| 李伟坤,洪海珊,白文芳.低频电磁场在移植骨髓源神经祖细胞修复脑损伤大鼠中的作用研究[J].中国康复医学杂志,2020,(10):1166~1171 |
| 低频电磁场在移植骨髓源神经祖细胞修复脑损伤大鼠中的作用研究 点此下载全文 |
| 李伟坤 洪海珊 白文芳 |
| 广州市增城区人民医院康复医学科，广州市，511300 |
| 基金项目：国家自然科学基金项目（81871842）； 广东省科技计划项目（2016A020214014） |
| DOI：10.3969/j.issn.1001-1242.2020.10.003 |
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| 摘要： |
| 摘要
目的：通过分析50Hz电磁场干预骨髓源神经祖细胞移植修复大鼠脑损伤后的脑组织病理，免疫荧光染色，行为学情况，探讨50Hz电磁场在骨髓源神经祖细胞移植修复大鼠脑损伤中的作用。
方法：应用自由落体法建立大鼠脑损伤模型，实验随机分为空白对照组、磁场组、细胞组和磁场+细胞组。移植细胞选用的是骨髓源神经祖细胞，采用贴壁法培养骨髓间充质干细胞P2，经悬浮诱导获得。造模术后7天，将Dil标记的骨髓源神经祖细胞移植至大鼠脑损伤部位，磁场干预组置于频率50Hz，强度5 mT正弦波电磁场中，60min/次，1次/天，共30天，空白对照组和细胞组大鼠处于无磁场刺激的相同环境中。在移植术后第1天、3天、7天、30天予Wayne Clark、爬梯试验行为学评分并取脑组织标本，采用HE染色观察大鼠脑组织病理变化；免疫细胞荧光检测移植细胞的胶质纤维酸性蛋白（GFAP）和神经元特异性微管蛋白（β-Ⅲ tubulin）的表达情况。
结果：移植术后30天，磁场+细胞组的Wayne Clark、爬梯试验评分显著高于其他组（P<0.01）；HE染色观察：磁场+骨髓源神经祖细胞组与其他组相比较，大鼠脑损伤部位水肿程度减轻，囊性空洞面积更小，细胞存活数较多；磁场+骨髓源神经祖细胞组大鼠损伤病灶的细胞免疫细胞荧光检测胶质纤维酸性蛋白阳性表达更少，神经元特异性微管蛋白阳性表达明显高于其他组。
结论：50Hz电磁场可促进骨髓源神经祖细胞移植修复脑损伤大鼠爬行功能恢复。 |
| 关键词：低频电磁场 骨髓源神经祖细胞 脑损伤 分化 |
| A study on low frequency electromagnetic fields on bone marrow-derived neural progenitor cells transplantion repairing the rats with brain injured Download Fulltext |
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| Department of Rehabilitation Medicine， Zengcheng District People's Hospital of Guangzhou， Guangzhou， 511300 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To analyze whether the alterations of brain tissue， immunofluorescence staining and behavioral score of rats with brain injured after the repairment of transplanting the bone marrow-derived neural progenitor cells (BM-NPCs) were related to the intervention of 50Hz electromagnetic fields. We therefore aimed to investigate the effects of 50Hz electromagnetic fields on enhancing the repairment of brain injured rats with transplantation of BM-NPCs.
Method： We established the model of brain trauma by the free-falling way. Rats were randomly divided into four groups：control group， BM-NPCs group，electromagnetic field group and electromagnetic field+BM-NPCs group. Each group was subdivided into 4 subgroups： 1-day， 3-day，7-day and 30-day of transplantation according to the observation points. We selected the BM-NPCs as the progenitors of transplanted cells which were obtained by culturing bone mesenchymal stem cells with the fast adherence method and by inducing under the suspension. BM-NPCs labeled with Dil were transplanted to the brain injury site of rats 7 days after the surgery. The electromagnetic field group was placed in a sine wave electromagnetic field 7 days after the surgery with the frequency of 50 Hz，the intensity of 5 mT for 60mins at a time per day，lasting for 30 days. Wayne Clark scale and the ladder rung walking test were performed to evaluate the behavior function and the pathology of brain tissues were observed using hematoxylin and eosin (HE) staining at each observation point. Furthermore， glial fibrillary acidic protein (GFAP) and Neuron-specific Class Ⅲ Beta-tubulin (β-Ⅲ tubulin) were detected using immune-fluorescence cytochemistry.
Result： After BM-NPCs transplantation for 30 days， the Wayne Clark scale and the ladder rung walking test of the electromagnetic field + BM-NPCs group were significantly higher than the other groups (P<0.01). HE staining showed that compared with the other groups， the brain edema degree was alleviated， the cystic void area was smaller and the number of nerve cells was increased in the electromagnetic field + BM-NPCs group. Moreover， the decrease of GFAP expression was observed in the electromagnetic field + BM-NPCs group， but the level of β-Ⅲtubulin expression was increased.
Conclusion： 50Hz electromagnetic fields could ameliorate the creeping disfunction in the brain injured rats after transplanting with BM-NPCs， and its mechanism might involve the induction of BM-NPCs to differentiate into neurons and elevation the expression of β-Ⅲ tubulin. |
| Keywords:low-frequency electromagnetic fields bone marrow-derived neural progenitor cells traumatic brain injured differentiation |
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