| 田明月,杨溢铎,覃琬婷,朱 晶,国海东,邵水金.雪旺细胞来源的外泌体miR-21通过靶向SPRY2促进大鼠坐骨神经损伤后修复的研究[J].中国康复医学杂志,2024,(6):767~774 |
| 雪旺细胞来源的外泌体miR-21通过靶向SPRY2促进大鼠坐骨神经损伤后修复的研究 点此下载全文 |
| 田明月 杨溢铎 覃琬婷 朱 晶 国海东 邵水金 |
| 上海中医药大学中西医结合学院人体解剖教研室，上海市，201203 |
| 基金项目：国家自然科学基金面上项目（82274633，81873357） |
| DOI：10.3969/j.issn.1001-1242.2024.06.002 |
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| 摘要： |
| 摘要
目的：研究高表达miR-21的雪旺细胞（Schwann cells，SC）来源外泌体对坐骨神经损伤（sciatic nerve injury，SNI）的修复作用及机制。
方法：分别采用空载慢病毒和高表达miR-21的慢病毒感染SC株，收集SC上清外泌体并进行鉴定。采用神经断端吻合术建立SNI大鼠模型，术后随机分为模型组、SC来源外泌体组和高表达miR-21的SC来源外泌体组（n=10）。其中，SC来源外泌体组和高表达miR-21的SC来源外泌体组分别采用体外收集的外泌体局部注射进行治疗，3周后行为学实验检测神经功能恢复，免疫荧光染色评价SNI后轴突髓鞘再生，RT-qPCR检测血清外泌体miR-21及神经组织中miR-21和SPRY2的表达水平，双荧光素酶报告基因实验体外验证miR-21和SPRY2之间的靶向互作。
结果：与模型组相比，其余各组大鼠坐骨神经功能和神经再生情况均出现明显改善，RT-qPCR结果显示血清外泌体及神经组织中miR-21的表达水平显著升高，神经组织中SPRY2的表达水平显著降低，其中高表达miR-21的SC细胞来源外泌体组较SC来源外泌体组变化更显著；双荧光素酶报告基因实验表明miR-21靶向调节SPRY2的表达。结论：高表达miR-21的SC来源外泌体通过靶向SPRY2显著促进SNI后修复。 |
| 关键词：雪旺细胞 外泌体 miR-21 坐骨神经损伤 功能恢复 神经再生 |
| Exosomal miR-21 derived from Schwann cells promoting the repair of sciatic nerve injury by targeting SPRY2 Download Fulltext |
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| Department of Anatomy， School of Basic Medicine， Shanghai University of Traditional Chinese Medicine， Shanghai， 201203 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To investigate the effect and potential mechanism of exosomal miR-21 derived from Schwann cell （SC） in the repairment of sciatic nerve injury （SNI）.
Method： SC was infected with negative control lentivirus and lentivirus that expression of miR-21， and its exosome was collected and identified respectively. The SNI rat model was established by nerve stump anastomosis. After surgery, the rats were randomly divided into the model group （n=10）， the SC-derived exosome group （n=10） and the SC-derived exosome group （n=10） that high expression of miR-21. The SC-derived exosomes group and SC-derived exosome group that high expression of miR-21 were treated with local injection of exosome collected in vitro. After 3 weeks， the functional recovery was detected by behavioral experiment. The nerve regeneration was evaluated by immunofluorescence staining. RT-qPCR was used to detect the expression of miR-21 in serum exosome and miR-21 and SPRY2 in sciatic nerve. The targeting interaction between miR-21 and SPRY2 was verified by dual-luciferase reporter gene experiment.
Result： Compared with the model group， the functional recovery and regeneration of sciatic nerve were significantly improved in the other two groups. The expression of miR-21 was significantly increased， while the expression of SPRY2 was significantly decreased. The improvement in the SC-derived exosome group with high expression of miR-21 were more significantly than those in the SC-derived exosome group.
Conclusion： Exosomal miR-21 derived from SC can significantly promote the repair of sciatic nerve injury by targeting SPRY2. |
| Keywords:Schwann cells exosome miR-21 sciatic nerve injury functional recovery nerve regeneration |
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