| 房金勇,李红玲,纪庆红,陈玉燕.高压氧对两种实验性脑出血大鼠模型出血灶周围水肿及水通道蛋白-4表达的影响[J].中国康复医学杂志,2012,27(11):987~992 |
| 高压氧对两种实验性脑出血大鼠模型出血灶周围水肿及水通道蛋白-4表达的影响 点此下载全文 |
| 房金勇 李红玲 纪庆红 陈玉燕 |
| 河北医科大学第二医院，石家庄，050000 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 2175 |
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| 摘要
目的:探讨高压氧（HBO）对两种实验性脑出血大鼠模型血肿周围水肿及水通道蛋白-4（AQP-4）表达的影响。
方法：将126只健康雄性SD大鼠随机分为5组：①假手术组6只（SHG）；②自体血诱导脑出血（A-ICH）对照一组30只；③胶原酶诱导脑出血（C-ICH）对照二组30只；④A-ICH模型加高压氧治疗（A-ICH+HBO）实验一组30只；⑤C-ICH模型加高压氧治疗（C-ICH+HBO）实验二组30只，后4组又随机分为术后24h、48h、72h、5d、7d五个时间点组，每组6只大鼠。高压氧治疗自术后24h开始，1次/d。于相应时间点将大鼠处死，进行脑水肿和AQP-4的测定。
结果: A-ICH模型成功率为65%,C-ICH模型成功率为75%。两对照组和两实验组大鼠脑组织含水量各时间点均高于假手术组，（A-ICH+HBO）经高压氧治疗后各时间点（24h、48h、72h、5d、7d）含水量均低于（A-ICH组），差异除24h组外均有显著性意义（P<0.05）。（C-ICH+HBO）经高压氧治疗后仅在ICH后48h和5d两个时间点与C-ICH对照组差异有显著性意义（P<0.05）。A-ICH+HBO和C-ICH+HBO组相比，前者水肿改善更早、更明显，尤其在72h时，差异显著（P<0.05）。脑出血后大鼠脑组织中AQP-4在各组均有表达，假手术组呈低表达状态，A-ICH和C-ICH两个对照组在脑出血后24h开始升高，48h达到高峰，72h后开始逐渐降低，7d时表达水平同假手术相近，但仍高于假手术组，A-ICH组与C-ICH组相比AQP-4表达更显著，但除72h组外其他时间点两组间无显著性差异（P>0.05）。（A-ICH+HBO）组各个时间点脑出血周围AQP-4的表达与A-ICH比较显著降低，差异具有显著性意义（P<0.05）。但（C-ICH+HBO）组AQP-4低表达仅在48h和72h与C-ICH组有差异（P<0.05）。A-ICH+HBO 组与C-ICH+HBO组相比AQP-4改善的较早、且明显，尤其在48h和72h差异显著（P<0.05）。
结论：HBO通过下调AQP-4的表达可有效减轻脑水肿。比较两种ICH模型发现，A-ICH模型在高压氧治疗中的敏感性更高，更适合高压氧在治疗脑出血水肿方面的研究。 |
| 关键词：高压氧 脑出血 脑水肿 水通道蛋白-4 |
| Effects of hyperbaric oxygen on brain edema and expression of aquaporin-4 surrounding brain tissue of hemorrhagic focus following experimental intracerebral hemorrhage in two kinds of rat models Download Fulltext |
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| Department of Rehabilitation, The Second Hospital of the Hebei Medical University, Shijiazhuang, 050000 |
| Fund Project: |
| Abstract: |
| Abstract
Objective:To study the effects of hyperbaric oxygen (HBO) on brain edema and expression of aquaporin-4(AQP-4) surrounding brain tissue of hemorrhagic focus following experimental intracerebral hemorrhage(ICH) in two kinds of rat models.
Method:A total of 126 rats were randomly divided into five groups:sham-operated group(SHG,6 rats);control-1 group(autologous blood induced intracerebral hemorrhage model,A-ICH,30 rats), control-2 group(collagenase-induced intracerebral hemorrhage model, C-ICH, 30 rats), trial-1 group(A-ICH+HBO,30 rats),trial-2 group(C-ICH+HBO,30 rats).HBO therapy was intervened at 24h after operation,once a day.All rats were sacrificed at 24h, 48h, 72h, 5d,7d post operation（each time point 6 rats）. Brain edema and expression of AQP-4 were tested.
Result:The success rates of A-ICH model and C-ICH model were 65% and 75% respectively. Brain edema content of A-ICH, C-ICH, A-ICH+HBO and C-ICH+HBO groups were significant higher than that of SHG group(P<0.05）.The cerebral edema of A-ICH+HBO and C-ICH+HBO groups alleviated obviously compared with A-ICH and C-ICH groups, and the difference was significant respectively at 48h,72h,5d,7d and 48h,5d post operation（P<0.05）. The brain edema of A-ICH+HBO group alleviated more early and obviously than that of C-ICH+HBO group, especially at 72h post operation(P<0.05）.The expression of AQP-4 appeared in all rats brain tissue,but expression of AQP-4 in SHG group was lower.That of A-ICH and C-ICH groups started rising at 24h post operation,the peak appeared at 48h,then dropped at 72h,but there were still significant different compared with SHG group at 7d post operation(P<0.05）.The difference between A-ICH group and C-ICH group was significant at 72h post operation(P<0.05），and the difference between A-ICH+HBO group and A-ICH group was significant at all time points post operation(P<0.05）. but the difference between C-ICH+HBO group and C-ICH group was significant only at 48h and 72h post operation(P<0.05）. Compared with C-ICH +HBO group, expression of AQP-4 in A-ICH+HBO group was more obvious and early,especially at 48h and 72h post operation(P<0.05）.
Conclusion:HBO might play neuroprotection role by relieving brain edema and down-regulating AQP-4 expression. A-ICH model was more fit for brain edema study of ICH rat. |
| Keywords:hyperbaric oxygen intracerebral hemorrhage brain edema aquaporin-4 |
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