潘良春,尹宗生,王伟,$2,高荣保,王明丽.新生和成年大鼠雪旺细胞培养、纯化的实验研究[J].中国康复医学杂志,2005,20(11):817~ |
新生和成年大鼠雪旺细胞培养、纯化的实验研究 点此下载全文 |
潘良春 尹宗生 王伟 $2 高荣保 王明丽 |
[1]安徽医科大学第一附属医院骨科,安徽合肥230022 [2]安徽医科大学微生物学教研室,安徽合肥230022 |
基金项目: |
DOI: |
摘要点击次数: 1966 |
全文下载次数: 155 |
摘要: |
目的:比较新生和成年大鼠雪旺细胞(Schwann cells,SCs)的体外培养和纯化方法。方法:分别取新生和成年SD大鼠的坐骨神经,用混合酶消化法、差速贴壁法和低浓度胰酶快速消化法进行培养和纯化,通过形态学观察和S-100免疫细胞化学鉴定,并行MTF法检测细胞增殖能力。结果:两组细胞生长状态良好,均呈S-100免疫细胞化学反应阳性,纯度均达95%以上;由生长曲线显示新生鼠SCs生长更快,第2代SCs倍增时间:新生鼠3d、成年鼠4d。结论:从新生和成年大鼠坐骨神经能成功分离培养出大量高纯度的SCs,新生鼠SCs增殖能力更强。 |
关键词:雪旺细胞 大鼠 坐骨神经 细胞培养 增殖 |
Experimental study of methods of culturing and purifying newborn and adult rat Schwann cells Download Fulltext |
PAN Liangchun YIN Zongsheng WANG Wei et al. |
Chinese Journal of Rehabilitation Medicine |
Fund Project: |
Abstract: |
Objective:To compare the methods of culturing and purifying newborn and adult rat SCs. Method: The sciatic nerves of newborn(aged 1-2 days) and adult(aged 3-4 months) SD rat were harvested. Trypsin and collagenase were used to separate SCs. Purifying SCs was obtained after a differential adhesion and low concentration trypsin in the course of passage. The purity of SCs was identified through the phase contrast microscope and S-100 immunocytochemical stain. The proliferating function of these SCs was assessed by MTT assay. Result: The cells in the two groups were in a good state and S-100-postive by immunocytochemistry, and the purity of SCs was more than 95%. The newborn SCs grew faster than the adult SCs. The doubling time of the 2nd passage of cells was 3 days for the newborn SCs and 4 days for the adult SCs. Conclusion:These findings indicate that a great number of highly purified SCs can be acquired from newborn and adult rat. The proliferation capacity of SCs isolated from newborn rat is higher than those from adult rat. |
Keywords:Schwann cells rat sciatic nerve cell culture proliferation |
|
查看全文 查看/发表评论 |