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徐梅,李红艳,徐波,耿传营,向青.鬼臼乙叉甙对肺癌A549细胞生长特性及Fas/FasL表达的影响[J].中国康复医学杂志,2006,21(3):237~239
鬼臼乙叉甙对肺癌A549细胞生长特性及Fas/FasL表达的影响    点此下载全文
徐梅  李红艳  徐波  耿传营  向青
中日友好医院临床医学研究所生化室,100029
基金项目:
DOI:
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摘要:
      目的:研究Vp16对人肺腺癌A549细胞生长特性及Fas/FasL表达的影响.方法:用MTT法检测细胞增殖特性,FCM观察细胞周期及凋亡,RT-PCR测定mRNA表达的变化.结果:Vp16以时间和剂量依赖方式作用于A549细胞的增殖;不同浓度的Vp16作用A549细胞,使其阻断在不同的细胞周期,且均能明显下调FasL的mRNA的表达(P<0.05),而对Fas mRNA的表达差异无显著性意义(P>0.05).结论:Vp16直接作用A549细胞主要引起细胞坏死,Vp16可能通过下调FasL的mRNA的表达,防止肿瘤细胞免疫逃逸,该结果为临床合理联合用药提供了实验依据.
关键词:肺癌 鬼臼乙叉甙 Fas/FasL系统 免疫逃逸 细胞凋亡
Effects of Vp16 on the growth characteristics and Fas/FasL expression on human carcinoma A549 cells    Download Fulltext
XU Mei  LI Hongyan  XU Bo  et al
Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, 100029
Fund Project:
Abstract:
      Objective:To investigate the effects of Vp16 on the growth characteristics and Fas/Fasl expression of A549 cells.Method:The growth rate, cell growth cycle and apoptosis were detected by MTT assay and FCM analysis respectively. The gene expression was determined by reverse transcription polymerase chain reaction(RT-PCR). Result:Vp16 inhibited the cell proliferation more effectively as dose and time increased. The cell cycle was arrested in dose-dependent manner, and FasLmRNA expression was dramatically decreased(P<0.05), meanwhile Fas mRNA expression was not significantly different(P>0.05) compared with the control. Conclusion:Vp16 cn mainly induce necrosis of human lung carcinoma A549 cells and prevent the immune escape of tumor cell by retarding FasLmRNA expression, which is valuable in selecting anti-cancer drugs in clinic therapy.
Keywords:lung carcinoma  VP16  Fas/FasL system  immune escape  apoptosis
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