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祝畅,张传汉,刘志恒,桂伶俐,高峰.人胚神经干细胞的长期贴壁培养和鉴定[J].中国康复医学杂志,2006,21(4):322~324
人胚神经干细胞的长期贴壁培养和鉴定    点此下载全文
祝畅  张传汉  刘志恒  桂伶俐  高峰
[1]华中科技大学同济医学院附属同济医院麻醉学教研室,武汉市解放大道1095号430030 [2]广东省深圳市第二人民医院麻醉科,武汉市解放大道1095号430030
基金项目:国家自然科学基金资助项目(30300328)
DOI:
摘要点击次数: 1942
全文下载次数: 1927
摘要:
      目的:探讨人胚神经干细胞的长期贴壁培养条件及传代方法.方法:在多种培养条件下,分别从9周和16周人胚脑中分离培养神经干细胞,应用免疫细胞化学的方法对所分离细胞的巢蛋白表达、自我更新能力和多向分化潜能进行鉴定.结果:采用高密度悬浮培养法能有效地从两种胚龄的人胚脑或室管膜下区中分离出神经干细胞,而采用贴壁培养法的人胚神经干细胞能保持干细胞的特性稳定增殖4个月.结论:从大小胚龄的人胚中均可成功分离培养人胚神经干细胞,贴壁培养的人胚神经干细胞能稳定增殖,是进一步进行人胚神经干细胞转基因研究的良好模型.
关键词:人胚胎 神经干细胞 细胞培养
Long-term adhere-wall culture and identification of human embryonic neural stem cell    Download Fulltext
ZHU Chang  ZHANG Chuanhan  LIU Zhiheng
Dept. of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030
Fund Project:
Abstract:
      Objective:To establish the method of long-term adhere-wall culture and passage of human embryonic neural stem cell.Method:By means of different culture methods, neural stem cells were isolated from human miscarriaged embryonic brain (embryonic age 9 weeks and 16 weeks). The capabilities of self-renew?nestin expression and multipotentiality of the neural stem cells were isolated and identified with immunocytochemical staining.Result:Human embryonic neural stem cell with the capabilities of self-renewing and multipotentiality can be isolated from the brain or subependyma of human embryo at different embryonic age by high concentration suspension culture. Neural stem cells possessed their characters and proliferated for 4 months stably by adhere-wall culture.Conclusion:Human embryonic neural stem cell can be isolated from human embryo of different embryonic age successfully. Human embryonic neural stem cells cultured by adhere-wall method can proliferate stably for a long time, which serve as a good model for transgenic research of human embryonic neural stem cell.
Keywords:human embryo  neural stem cell  cell culture
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