设为首页
加入收藏
联系我们
Email-Alert
 

    首页 | 杂志介绍 | 编委成员 | 投稿指南 | 订阅指南 | 过刊浏览 | 论著模板 | 综述模板 | 帮助

 
鲍春龄,东红升,东贵荣.头穴透刺对脑出血大鼠脑组织GLUT1表达的影响[J].中国康复医学杂志,2007,22(12):1067~1069
头穴透刺对脑出血大鼠脑组织GLUT1表达的影响    点此下载全文
鲍春龄  东红升  东贵荣
上海中医药大学附属岳阳中西医结合医院针灸科,上海,200437
基金项目:国家自然基金资助课题(30500677),黑龙江省研究生创新基金资助课题(YJSCX2005-214HLJ)
DOI:
摘要点击次数: 1795
全文下载次数: 2087
摘要:
      目的:研究大鼠脑出血后血肿周围组织葡萄糖载体蛋白-1(GLUT1)表达的动态变化规律及头穴透刺的治疗作用。方法:将雄性Wistar大鼠随机分为空白组、假手术组、模型组、针刺组,每组又分为24h、72h、168h三个小组,采用胶原酶/肝素联合注入法制备脑出血大鼠模型,给以百会透太阳穴电针治疗,各组动物在指定时间断头取脑,取血肿周围3mm范围内组织,采用免疫组织化学法观测GLUT1表达的变化。结果:模型组:三个时间点均见大部分GLUT1阳性微血管形态变窄、皱缩、闭塞,越靠近病灶越严重;GLUT1表达24h先代偿性升高,与假手术组比较P<0.05,后72h、168h失代偿性降低,与假手术组比较P<0.05;针刺组:仅在24h 时血肿周围出现了轻微的管腔狭窄,之后大部分血管表现为管腔呈圆形扩张, GLUT1在72h、168h也出现下降(P<0.05),但明显好于模型组(P<0.05)。结论:针刺在多时间点对GLUT1均有治疗作用,能延长治疗时间窗,减轻病变程度且早期作用明显。
关键词:头针  脑出血  葡萄糖载体蛋白-1
Experimental study of scalp-acupuncture on the express of GLUT1 after rat intracerebral hemorrhage    Download Fulltext
The Affiliated Yueyang Hospital of Integrated Traditional Chinese and Western Medicine; Shanghai University of TCM; Shanghai, 200437
Fund Project:
Abstract:
      Objective: To prove the effect of acupuncture through an experimental study of scalp-acupuncture on the express of GLUT1 after intracerebral hemorrhage in rats. Method: Wistar rats were divided into 4 groups: normal group, sham group, model group, acupuncture group. The last three group was divided into three time groups(24h,72h,168h), The model of intracerebral hemorrhage in rat was made with Collagenase Ⅶ/heparini infusion, and treated with electro-acupuncture of Baihui-Taiyang points, Immunohistochemical method used to observe the expression of GLUT1 around the hemotoma. Result: The model group after intracerebral hemorrhage: morphology of majority positive GLUT1 capillary vessels showed narrowing, shrinking and obliteration in three time points specially near the focus of lesion. The expression of GLUT1 in 24h revealed a compensatory rising at first, compared with control group(P<0.05); then lowered, in 72h and in 168h (P<0.05); Acupuncture group: in perihemotoma area, the morphology of positive GLUT1 capillary vessels showed slightly narrowing only in 24h, then most of the vessels showed spherical distending, GLUT1 decreased in 72h and 168h (P<0.05),but better than the model group(P<0.05). Conclusion: Acupuncture have therapeutic effects on three time points, delay the development of pathologic change in time,lengthen the therapy time window, and obviously effect in the earlier period.
Keywords:scalp acupuncture  intracerebral hemorrhage  glucose transporter proteins
查看全文  查看/发表评论

您是本站第 37841838 位访问者

版权所有:中国康复医学会
主管单位:中国科学技术协会 主办单位:中国康复医学会
地址:北京市朝阳区樱花园东街,中日友好医院内   邮政编码:100029   电话:010-64218095   

本系统由北京勤云科技发展有限公司设计
京ICP备18060696号-2

京公网安备 11010502038612号