| 鲍春龄,东红升,东贵荣.头穴透刺对脑出血大鼠脑组织GLUT1表达的影响[J].中国康复医学杂志,2007,22(12):1067~1069 |
| 头穴透刺对脑出血大鼠脑组织GLUT1表达的影响 点此下载全文 |
| 鲍春龄 东红升 东贵荣 |
| 上海中医药大学附属岳阳中西医结合医院针灸科，上海，200437 |
| 基金项目：国家自然基金资助课题（30500677），黑龙江省研究生创新基金资助课题（YJSCX2005-214HLJ） |
| DOI： |
| 摘要点击次数: 2020 |
| 全文下载次数: 2223 |
| 摘要： |
| 目的：研究大鼠脑出血后血肿周围组织葡萄糖载体蛋白-1（GLUT1）表达的动态变化规律及头穴透刺的治疗作用。方法：将雄性Wistar大鼠随机分为空白组、假手术组、模型组、针刺组，每组又分为24h、72h、168h三个小组，采用胶原酶/肝素联合注入法制备脑出血大鼠模型，给以百会透太阳穴电针治疗，各组动物在指定时间断头取脑，取血肿周围3mm范围内组织，采用免疫组织化学法观测GLUT1表达的变化。结果：模型组：三个时间点均见大部分GLUT1阳性微血管形态变窄、皱缩、闭塞，越靠近病灶越严重；GLUT1表达24h先代偿性升高，与假手术组比较P<0.05，后72h、168h失代偿性降低，与假手术组比较P<0.05；针刺组：仅在24h 时血肿周围出现了轻微的管腔狭窄，之后大部分血管表现为管腔呈圆形扩张， GLUT1在72h、168h也出现下降（P<0.05），但明显好于模型组（P<0.05）。结论：针刺在多时间点对GLUT1均有治疗作用，能延长治疗时间窗，减轻病变程度且早期作用明显。 |
| 关键词：头针 脑出血 葡萄糖载体蛋白-1 |
| Experimental study of scalp-acupuncture on the express of GLUT1 after rat intracerebral hemorrhage Download Fulltext |
|
| The Affiliated Yueyang Hospital of Integrated Traditional Chinese and Western Medicine; Shanghai University of TCM; Shanghai, 200437 |
| Fund Project: |
| Abstract: |
| Objective: To prove the effect of acupuncture through an experimental study of scalp-acupuncture on the express of GLUT1 after intracerebral hemorrhage in rats. Method: Wistar rats were divided into 4 groups: normal group, sham group, model group, acupuncture group. The last three group was divided into three time groups(24h,72h,168h), The model of intracerebral hemorrhage in rat was made with Collagenase Ⅶ/heparini infusion, and treated with electro-acupuncture of Baihui-Taiyang points, Immunohistochemical method used to observe the expression of GLUT1 around the hemotoma. Result: The model group after intracerebral hemorrhage: morphology of majority positive GLUT1 capillary vessels showed narrowing, shrinking and obliteration in three time points specially near the focus of lesion. The expression of GLUT1 in 24h revealed a compensatory rising at first, compared with control group(P<0.05); then lowered, in 72h and in 168h (P<0.05); Acupuncture group: in perihemotoma area, the morphology of positive GLUT1 capillary vessels showed slightly narrowing only in 24h, then most of the vessels showed spherical distending, GLUT1 decreased in 72h and 168h (P<0.05)，but better than the model group(P<0.05). Conclusion: Acupuncture have therapeutic effects on three time points， delay the development of pathologic change in time，lengthen the therapy time window, and obviously effect in the earlier period. |
| Keywords:scalp acupuncture intracerebral hemorrhage glucose transporter proteins |
|
| 查看全文 查看/发表评论 |
|
|
|
|
|
