| 邱 峰,励建安,陆 晓,黄 澎,吴 涛,孟祥博,高 晶,刘 鹏.心肌缺血和有氧运动训练诱导VEGF表达时间规律的实验研究[J].中国康复医学杂志,2008,23(3):193~197 |
| 心肌缺血和有氧运动训练诱导VEGF表达时间规律的实验研究 点此下载全文 |
| 邱 峰 励建安 陆 晓 黄 澎 吴 涛 孟祥博 高 晶 刘 鹏 |
| 南京医科大学第一附属医院康复医学科,南京, 210029 |
| 基金项目：国家自然科学基金资助项目(30370687) |
| DOI： |
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| 摘要： |
| 摘要 目的：研究心肌缺血和有氧运动训练诱导血管内皮细胞生长因子（VEGF）表达的时间规律。方法：成功建模的健康成年巴马小型猪24只，体重23.7±4.0kg，随机分为对照组、缺血组和运动组。将可控性水囊梗阻器安装在猪冠状动脉钝缘支处，建立可控性猪心肌缺血模型。对照组不进行缺血刺激和有氧运动训练；缺血组进行单纯缺血刺激，2min/次，2次/d，持续缺血刺激8周；运动组除进行缺血刺激外，每天进行一次平板有氧运动训练，每次30min。每两周取动脉血，采用ELISA法检测血液VEGF。实验终点时采用Western Blot检测缺血区心肌VEGF。结果：缺血组第2周血液中VEGF的表达量明显高于实验前（P<0.05），随后进入平台期（P>0.05）。运动组中第2-4周血液中VEGF的表达量持续增高（P<0.05），随后达到平台期(P>0.05)。缺血区心肌VEGF表达和血液VEGF表达呈正相关（r=0.826，P<0.01）。结论：心肌缺血和有氧运动训练诱导的血液VEGF增高分别在第2周和第4周达到高峰，然后进入平台期。
关键词 心肌缺血;有氧运动;血管内皮细胞生长因子
中图分类号：R493,R541.4 文献标识码：A 文章编号：1001-1242(2008)-03-0193-05 |
| 关键词：心肌缺血 有氧运动 血管内皮细胞生长因子 |
| Temporal phenomenon of myocardial ischemia and aerobic exercises-induced VEGF expression: an experimental study Download Fulltext |
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| Dept. of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University, Jiangsu, Nanjing, 210029 |
| Fund Project: |
| Abstract: |
| Objective:To study the temporal phenomenon of myocardial ischemia and aerobic exercises training induced expression of vascular endothelial growth factor(VEGF) in swine. Method:Twenty-four miniature swines were randomly assigned to control group(CG), ischemia group(IG) and exercises group(EG). A controllable hydraulic balloon catheter was placed at the first marginal branch of left circumflex coronary artery. A model of controllable myocardial ischemia was established. Subjects in IG and EG received transient coronary occlusion for 2min repeated twice a day for 8weeks. Subjects in EG received 30 min aerobic exercises training on treadmill with intermittent high intensity 2min duration which repeated twice a session. Blood samples were obtained every 2 weeks. The ischemic area of the myocardium was also sampled after the completion of 8-week experiment. VEGF in serum were analyzed by ELISA and VEGF in myocardium was measured by Western Blot. Result: VEGF serum level on week 2 in IG was significantly higher than that in CG (P<0.05) and then reached plateau (P>0.05). EG demonstrated the significant increase of VEGF from baseline to week 4 (P<0.05), and then reached the plateau (P>0.05). VEGF in serum was positively correlated that in myocardium(r=0.826, P<0.01). Conclusion: Intermittent myocardial ischemia and aerobic exercises training induced VEGF expression reaches peak on week 2 and week 4 respectively, and then reaches plateau. |
| Keywords:myocardial ischemia aerobic exercises training vascular endothelial growth factor |
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