| 赵伦华,程少华,王 珏,张 健,朱大和.高压氧对兔早期激素性股骨头缺血性坏死骨修复的影响[J].中国康复医学杂志,2008,23(4):339~340 |
| 高压氧对兔早期激素性股骨头缺血性坏死骨修复的影响 点此下载全文 |
| 赵伦华 程少华 王 珏 张 健 朱大和 |
| 郧阳医学院附属太和医院病理科，湖北省十堰市，442000 |
| 基金项目：湖北省教育厅重点项目（D200524003） |
| DOI： |
| 摘要点击次数: 2238 |
| 全文下载次数: 2004 |
| 摘要： |
| 摘要 目的：建立激素性股骨头缺血性坏死（SANFH)动物模型，然后采用高压氧（HBO)治疗，探讨HBO治疗早期SANFH的病理变化及作用机制。方法： 健康成年日本大耳白兔60只，随机分为模型组(n=42)与对照组(n=18)，造模成功后，再将模型组分为HBO组(n=16)及其对照组(n=16)。观察模型组、HBO组及其各自对照组X线、MRI、光镜、透射电镜的变化。结果：①模型组第6周股骨头软骨下区骨小梁稀疏、变细，甚至骨小梁断裂，死骨形成，部分关节软骨区域性坏死。髓腔内造血组织减少，肥大脂肪细胞增多，后期脂肪细胞液化坏死，基质水肿出血。透射电镜观察到骨细胞坏死及成骨细胞凋亡现象。②HBO组第4—6周时不同程度的造血功能恢复，肥大脂肪细胞数目逐渐减少，部分骨小梁周围出现少量梭形或成排的成骨细胞，髓腔内纤维组织增生，在部分坏死的关节软骨下区髓腔内见活跃的纤维组织及成骨细胞增生；第8周时坏死骨小梁周围出现多核破骨细胞及较肥胖的成骨细胞，造血细胞增生明显、肥大脂肪细胞明显减少。透射电镜观察到成骨细胞、骨细胞、胶原纤维再生、修复的证据。结论： HBO治疗对早期SANFH有明显的骨修复作用。
关键词 股骨头坏死；激素；高压氧；修复；动物模型；兔
中图分类号:R361，R459.6 文献标识码:A 文章编号:1001-1242(2008)-04-0339-02
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| 关键词：股骨头坏死 激素 高压氧 修复 动物模型 兔 |
| Effects on steriod-induced avascular necrosis of femoral head during the early phase of reparative process of bone by hyperbaric oxygenation in rabbits Download Fulltext |
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| Department of Pathology, Taihe Hospital, Yunyang Medical College, 442000 |
| Fund Project: |
| Abstract: |
| Objective: To establish an animal model of steroid-induced avascular necrosis of femoral head(SANFH),and treated with hyperbaric oxygen(HBO). Thus to investigate the pathological changes and the mechanism of the treatment of SANFH in early stage through HBO therapy. Method: Sixty mature Japanese rabbits were randomly divided into two groups: model group(n=42) and control group(n=18). After succeeded in establishment, they were subdivided into HBO group(n=16) and its control group(n=16). The changes of X-ray, MRI and histopathological changes were observed with light microscopy and electron microscopy in all groups. Result: ①After six weeks in model group, thinned and sparse of trabecula of bones，parts of trabecula of bone in the formal head under cartilage ruptured and dead bones developed. Partial joint cartilage necrotized were found microscopicly in femoral head under cartilage, hematopoietic tissue decreased and fat cells increased and followed with liquefactive necrosis. Bone marrow became edema and hemorrhage. Evidence of necrosis of osteocyte and apoptosis of osteoblasts were found by electron microscopy. ②In HBO group, function of haematogenous tissue recovered in various degrees in the 4th to 6th week. Number of fat cells reduced. A small amount of spindly osteoblasts in a row appeared around few bone trabecula. Fibrous tissue and osteoblasts in the marrow under parts of necrotized joint cartilage actively proliferated. In the 8th week, osteoclasts and round osteoblast appeared around necrotized trabecula of bone. Haematogenous cells proliferated significantly and enlarged fat cells decreased markedly. Evidences of regeneration and repair of osteoblasts, osteocytes and collagen were found by electron microscopy. Conclusion: Hyperbaric oxygen therapy can obviously promote repair of bone in SANFH in early stage. |
| Keywords:avascular necrosis of femoral head hormone hyperbaric oxygen repair animal model rabbit |
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