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代喻兵,骆文龙,陈红江,聂江华,方 黎,赖晓霏,李晶兢.面神经损伤后rhEPO对面运动神经元的保护作用[J].中国康复医学杂志,2008,23(6):491~494
面神经损伤后rhEPO对面运动神经元的保护作用    点此下载全文
代喻兵  骆文龙  陈红江  聂江华  方 黎  赖晓霏  李晶兢
重庆医科大学附属第二医院耳鼻咽喉-头颈外科,重庆, 400010
基金项目:
DOI:
摘要点击次数: 2456
全文下载次数: 1962
摘要:
      目的:观察重组人促红细胞生成素(recombinant human erythropoietin, rhEPO)对大鼠受损面神经的保护作用及对Caspase-3表达的影响。方法:75只雌性大鼠随机分为rhEPO治疗组(n=25) 、对照组(n=25)、假手术组(n=25),50只大鼠建立左侧面神经干损伤动物模型,rhEPO组大鼠致伤后即刻及每天腹腔内注射rhEPO(5000U/kg),连续两周,对照组给予等量生理盐水。分别于第3、7、14、21、28天采用Toluidine blue染色计算面神经元存活率,TUNEL检测细胞凋亡,免疫组化检测Caspase-3的表达。结果:从伤后第7天开始治疗组和对照组左侧面神经元存活率逐渐下降,每个时间点治疗组面神经元存活率明显高于对照组(P<0.05);治疗组与对照组在伤后第3天未见Tunel染色阳性细胞,对照组第7天见表达,第14天数量达高峰,治疗组在伤后第7、14、21天面神经元凋亡细胞数显著减少 (P<0.05);伤后第3天对照组见 Caspase-3表达增加,第14天达高峰,第28天仍见少许表达,治疗组在各时间点Caspase-3表达均明显低于对照组(P<0.05)。结论:rhEPO对受损大鼠面神经元有保护作用;降低Caspase-3的表达、抑制细胞凋亡可能是rhEPO治疗创伤性面瘫的重要机制。
关键词:面神经  运动神经元  促红细胞生成素,重组  凋亡
Neuroprotective effect of rhEPO on facial motoneurons after facial nerve injury in rats    Download Fulltext
Department of Otorhinolaryngology-Head & Neck Surgery,The Second Affiliated Hospital of Chongqing Medical University,Chongqing, 400010
Fund Project:
Abstract:
      Objective:To observe the neuroprotective effect of rhEPO on injuried facial motoneurons(FMNs) and its impact on the expression of Caspase-3. Method:The rat modeIs of facial nerve injury were established by crashing main trunk of left facial nerve. Seventy-five female rats were randomly divided into 3 groups: rhEPO treatment group(treated with rhEPO 5000U/kg ip once a day following injury for two weeks,n=25), control group(treated with the same dose of normal saline ip once a day following injury for two weeks,n=25),sham operation group(n=25).Rats were sacrificed on the 3rd, 7th, 14th, 21st, 28th d after injury. The survival rates of FMNs after facial nerve injury were studied by Toluidine blue staining. The apoptotic cells of injured FMNs were detected by TUNEL staining.The expression of Caspase-3 were detected by immunohistochemistry method. Result: The survival rates of FMNs both in rhEPO treatment group and control group decreased gradually from the 7th d to 28th d, the survival rates of FMNs at each time point in rhEPO treatment group were significantly higher than that in control group(P<0.05). TUNEL-positive cell wasn′t observed on the 3rd d after injury both in rhEPO treatment group and control group,but was noted on the 7th d after injury and it′s number peaked on the 14th d in control group. The apoptotic cell numbers in the rhEPO treatment group were significantly lower than that in control group from 7d to 21d(P<0.05). The expression of Caspase-3 increased 3d after injury and peaked at 14d in control group. The expression level of Caspase-3 in rhEPO treatment group was significantly lower than that in control group at each time point(P<0.05).Conclusion: rhEPO can effectively protect facial motoneurons and reduce the expression of Caspase-3 after facial nerve injury; inhibition of apoptosis and decreased expression of Caspase-3 by rhEPO may be an important mechanism for treatment of traumatic facial paralysis.
Keywords:facial nerve  motoneurons  eythropoietin  recombinant  apoptosis
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