| 汪 薇,张立新,张志强,苑秀华.不同物理因子对大鼠脑缺血再灌注损伤的保护作用的研究[J].中国康复医学杂志,2008,23(8):694~696 |
| 不同物理因子对大鼠脑缺血再灌注损伤的保护作用的研究 点此下载全文 |
| 汪 薇 张立新 张志强 苑秀华 |
| 中国医科大学实验技术中心, 沈阳， 110001 |
| 基金项目：辽宁省教育厅科学技术研究项目(20061007) |
| DOI： |
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| 摘要： |
| 目的:通过观察不同物理因子对大鼠脑缺血再灌注后脑梗死体积,脑组织中B细胞淋巴细胞瘤-xl及肿瘤坏死因子-α表达的影响,比较不同物理因子对脑缺血再灌注损伤后的保护作用并初步探讨其作用机制。方法:用线栓法制备一侧大脑中动脉栓塞再灌注大鼠模型,造成大鼠右脑缺血2h再灌注24h,采用Zea-Longa 5级评分法评定神经功能缺损程度来筛选实验用鼠。大鼠按体重随机分成假手术组、对照组及超短波组,电刺激组,旋磁组。所选大鼠均于再灌注24h后断头取脑，分别测定脑梗死灶体积、脑组织中Bcl-xl及TNF-α的表达。结果:与对照组比较，超短波组及电刺激组的梗死体积均缩小，差异有显著性意义（P<0.05）,而旋磁组未见梗死体积的显著缩小。与对照组比较，各治疗组脑组织中Bcl-xl表达增加, TNF-α水平降低，均具有显著性意义（P<0.05）。结论:超短波及电刺激治疗均可通过增加Bcl-xl表达降低TNF-α水平而抑制脑神经细胞凋亡，挽救半暗带，缩小脑梗死灶体积，从而保护脑缺血再灌注后神经损伤，而旋磁治疗抑制凋亡的作用明显，而缩小梗死体积的作用不明显可能与实验动物数量少有关。 |
| 关键词：超短波 电刺激 旋磁 脑缺血再灌注损伤 作用机制 |
| Study of comparing the protective effects of different physical modalities on cerebral ischemia-reperfusion injury of rats Download Fulltext |
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| Experimental Technology Center, China Medical University, Shenyang, 110001 |
| Fund Project: |
| Abstract: |
| Objective: To observe the effects of different physical modalities on infarction volume,the expressions of B cell lymphocytoma-xl(Bcl-xl) and tumor necrosis factor alpha(TNF-α) after cerebral ischemia-reperfusion injury in rats and to compare their protective effects and discuss their action mechanisms. Method: Focal ischemia-reperfusion model was established in rats with reversible right middle cerebral artery occlusion with filament. Right cerebral ischemia was for 2 hours and then with 24 hours reperfusion. The severity of neurological deficits were evaluated by Zea-Longa 5-grade scoring to select experimental rats. After surgery,the rats were divided into 5 groups:sham operation group, control group,ultrashortwave treatment group, electrical stimulation treatment group and rotated magnetic field treatment group. Result: Forty-five rats were involved in the analysis of results:①Compared with control group, in ultrashortwave treatment group and electrical stimulation treatment group the infarcton volume reduced, the differences were significant（P<0.05）, but in rotated magnetic field treatment group that was not. ②Compared with control group, the exprssions of Bcl-xl in all treatment groups elevated, but the expressions of TNF-α reduced, the differences were significant（P<0.05）.Conclusion:Ultrashortwave and electrical stimulation therapy can protect cerebra from ischemia-reperfusion injuries and enhance the therapeutic effects by elevating the expression of Bcl-xl and reducing the level of TNF-α to inhibit neuron apoptosis, saving ischemic penumbra and reducing infarction volume. The rotated magnetic field therapy has significant effect on inhibiting neuron apoptosis. |
| Keywords:ultrashortwave electrical stimulation rotated magnetic field cerebral ischemia-reperfusion injury action mechanism |
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