| 李红玲,葛艳萍,刘春辉,岳 崴,曹慧芳.运动训练对出血性脑损伤GAP-43基因及蛋白表达的影响[J].中国康复医学杂志,2008,23(10):884~887 |
| 运动训练对出血性脑损伤GAP-43基因及蛋白表达的影响 点此下载全文 |
| 李红玲 葛艳萍 刘春辉 岳 崴 曹慧芳 |
| 河北医科大学第二医院康复科，石家庄，050000 |
| 基金项目：河北省卫生厅科研基金项目（07042） |
| DOI： |
| 摘要点击次数: 2226 |
| 全文下载次数: 2017 |
| 摘要： |
| 目的：观察运动训练对脑出血大鼠生长相关蛋白（GAP-43）基因及其蛋白表达的影响。方法：实验动物用健康雄性SD大鼠160只。先将96只大鼠随机分为3组，实验组（出血后运动，n=32）、对照组（出血后不运动，n=32）、假手术组（无出血不运动，n=32）。各组又分为术后第7天，第14天，第21天，第28天共4个时相点，每个时相点4只用于免疫组化检测，4只用于RT-PCR检测。实验组大鼠于术后第72小时开始跑笼训练，其余大鼠在标准笼内自由活动。再将另外64只随机分为脑出血后第6小时，第12小时，第24小时， 第48小时，第72小时，第7天，假手术组和正常组，每组4只分别用于免疫组化和RT-PCR检测。结果：①免疫组化结果：GAP-43阳性细胞表达为细胞胞浆着棕黄色，阳性细胞主要分布于血肿周围和大脑皮质的神经元，实验组于脑出血后第12小时开始上调，持续到第7天，与正常组和假手术组相比差异有显著性（P<0.05）。实验组于运动后第14天出现上调，第28天达高峰，与对照组相比差异有显著性（P<0.05），实验组和对照组与假手术组相比差异有显著性（P<0.01）。②RT-PCR结果：脑出血后第12小时GAP-43 mRNA 表达一度升高，与对照组比差异有显著性（P<0.05），之后维持较高水平，直到第14—28天。实验组表达于第14—28天与假手术组相比差异有显著性（P<0.05），与对照组比（P>0.05）。结论：GAP-43参与了脑出血后神经可塑性的发生，运动训练可促进GAP-43基因及蛋白表达，从而改善神经功能。 |
| 关键词：运动训练 脑出血 大鼠 生长相关蛋白-43 |
| The effects of exercises on expression of GAP-43 and GAP-43mRNA after ICH in rats Download Fulltext |
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| Department of rehabilitation, The Second Hospital of Hebei Medical University,Shijiazhuang, 050000 |
| Fund Project: |
| Abstract: |
| Objective: To investigate the effects of exercises on expression of GAP-43(growth associated protein-43) and GAP-43mRNA of neuronal cells after intracerebral hemorrhage(ICH) in rats. Method： Ninety-six male SD rats(body weight, 270—300g) were divided into three groups, trial group(ICH and exercises group, n=32), control group(ICH group, n=32) and sham operated group (no ICH without exercises, n=32). The rats brains were removed at the 7th d, 14th d, 21st d, 28th d after operation. The other 64 rats were divided into 8 groups(the 6th h, 12th h,24th h,48th h,72th h,7th d after ICH, sham operated and normal). The activations of GAP-43 and GAP-43mRNA were measured by immunohistochemistry and RT-PCR. The rats in trial group began cage-running exercises at the 72nd h after operation. The others lived in standard cages. Result： ①The result of immunohistochemistry: GAP-43-positive cells distributed around the hematoma and neuron of cortex. The number of GAP-43 -positive cells was very little in sham-operation group. In trial group up-regulation of GAP-43 displayed at the 12th h after ICH, until the 7th d after ICH, and there was significant difference compared with sham operated group and normal group（P<0.05）. There was up-regulation of expression of GAP-43 in trial group at the 14th d-28th d after operation, and there was significant difference in trial group and control group compared with sham operated group（P<0.01）, but in trial group the expression of GAP-43 was on a higher level than that in control group（P<0.05）. ②The result of RT-PCR: There was up-regulation of GAP-43mRNA at the 12th h after ICH in trial group, compared with control group(P<0.05). In trial group and control group there was higher expression at the 14th—28th d than that in sham-operated group(P<0.05), but in trial group there was no significant difference compared with control group (P>0.05). Conclusion：The results suggested that GAP-43 participated in neuronal plasticity after ICH, and exercises training could clearly up-regulate the expression of GAP-43 and GAP-43-mRNA and improve neurological function. |
| Keywords:exercise intracerebral hemorrhage rats growth associated protein-43 |
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