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陶 静,陈立典,薛偕华,杨珊莉.电针对局灶性脑缺血成年大鼠神经干细胞增殖、分化的影响[J].中国康复医学杂志,2008,23(12):1061~1063
电针对局灶性脑缺血成年大鼠神经干细胞增殖、分化的影响    点此下载全文
陶 静  陈立典  薛偕华  杨珊莉
福建中医学院2006级博士,350003
基金项目:国家自然科学基金(30672731)
DOI:
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摘要:
      目的:观察电针对成年大鼠局灶性脑缺血后神经干细胞增殖、分化的影响,探讨其治疗脑梗死的可能机制。方法:采用线栓法制备大鼠MCAO模型,应用BrdU/NeuN、BrdU/GFAP免疫荧光双标法观察再灌注损伤后第4、7、14天时大鼠神经干细胞增殖、分化情况。结果:脑缺血后第4、7、14天3个时间点室管膜下区、缺血边缘区均有BrdU阳性细胞表达,BrdU阳性细胞数在第7天达高峰;电针组第7天BrdU阳性细胞数高于相同时间段模型组(P<0.05);电针组BrdU/GFAP双标阳性细胞数在7天高于相同时间段模型组(P<0.05);电针组和模型组BrdU/NeuN双标阳性细胞数在缺血后第4、7和14天差异无显著性(P>0.05)。结论:缺血损伤可诱发室管膜下区、缺血边缘区神经干细胞的增殖,少量的新增殖细胞可以向神经元和星型胶质细胞分化;电针可以促进脑缺血后细胞增殖作用并可激发新增殖细胞向星型胶质细胞分化,而对向神经元细胞分化则影响不明显。
关键词:大鼠  局灶性脑缺血  电针  神经干细胞
Effects of electroacupuncture on proliferation and differentiation of nerve stem cells in the adult rats after brain ischemic injury    Download Fulltext
Fujian University of TCM, 350003
Fund Project:
Abstract:
      Objective: To study the effects of electroacupuncture on proliferation and differentiation of neural stem cells(NSC), and to explore the possible mechanism of electroacupunctue in treatment of ischemic stroke. Method: The model of acute cerebral ischemia reperfusion injury in rat was made by means of middle cerebral artery occlusion. All animals were sacrificed after different durations of reperfusion (4、7、14d). The double-label immunofluorescence histochemistry method with BrdU/NeuN or BrdU/GFAP was used to observe the changes of cell proliferation and differentiation in adult rat brains. Result: BrdU+ cells were found in subependymal region and ischemic marginal zone at different time points after cerebral ischemia. The level of BrdU+ cells proliferation peaked at the 7thd. The number of BrdU-positive cells in electro-acupuncture group was more than that in model group at same time(P<0.05). Compared with model group, the number of BrdU/GFAP positive cells in electro-acupuncture group increased significantly on the 7th d (P<0.05). There was no significant difference on the number of BrdU/NeuN positive cells between electro-acupuncture group and model group(P>0.05). Conclusion: Ischemic injury can induce the proliferation of NSC in subependymal region and ischemic marginal zone. Electro- acupuncture can promote proliferation of NSC and stimulate it differentiate further into astrocytes. It may be one of the important mechanisms of electro acupuncture in treating ischemic stroke.
Keywords:rat  focal cerebral ischemic injury  electroacupuncture  neural stem cell
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