| 李 平,姚文龙,钱 巍,张传汉.脊髓损伤后大鼠Cdh1 mRNA表达的变化[J].中国康复医学杂志,2009,24(01):12~14 |
| 脊髓损伤后大鼠Cdh1 mRNA表达的变化 点此下载全文 |
| 李 平 姚文龙 钱 巍 张传汉 |
| 华中科技大学同济医学院附属同济医院麻醉学教研室, 湖北武汉, 430030 |
| 基金项目：国家自然科学基金资助项目（30571788） |
| DOI： |
| 摘要点击次数: 2586 |
| 全文下载次数: 2022 |
| 摘要： |
| 目的: 探讨大鼠脊髓损伤(SCI)后损伤区脊髓组织Cdh1 mRNA的表达变化。方法: 32只雄性SD大鼠随机分成对照组（C组）、SCI组（M组），每组16只。M组采用改良Allen打击法建立SCI模型；C组行假手术，仅暴露脊髓。术后第l、3、5、7天对大鼠后肢运动功能采用Basso-Beattie-Bresnahan(BBB)评分进行评估；取损伤节段脊髓，提取组织总RNA，采用实时荧光定量PCR检测损伤区脊髓组织Cdh1 mRNA的表达。结果:术后各时点M组BBB评分均低于C组(P<0.05)。术后各时点C组Cdh1 mRNA表达比较， 差异无显著性意义，M组Cdh1 mRNA 表达逐渐降低(P<0.05) 。与C组相比，M组术后第1天Cdh1 mRNA的表达增加 (P<0.05), 术后第5 、7 天Cdh1 mRNA的表达减少。结论:大鼠SCI后损伤区脊髓组织Cdh1 mRNA表达降低，提示APC-Cdh1可能参与SCI后的病理生理过程。 |
| 关键词：脊髓损伤 细胞周期后期分裂促进复合物 基因表达 |
| Changes of expression of Cdh1 mRNA in injured myeloid tissue after spinal cord injury in rats Download Fulltext |
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| Dept. of Anesthesiology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan,430030,China |
| Fund Project: |
| Abstract: |
| Objective: To investigate the changes of expression of Cdh1 mRNA in injured myeloid tissue after spinal cord injury（SCI）in rats. Method: Thirty-two adult SD rats were randomly divided into two groups：sham group（group C）and SCI group（group M）. SCI model was established with modified Allen′s crush method in rats. The hind limb movement was assessed with Basso-Beattie-Bresnahan(BBB) scales and mRNA of injured myeloid tissue was extracted for Cdh1 examination with real time PCR at the 1st,3rd,5th,7th d after SCI． Result:The BBB scores in group M were significantly lower than that in group C at the 1st,3rd,5th,7th d respectively after SCI(P<0.05). The expression of Cdh1 mRNA in group M at the 1st,3rd,5th,7th d decreased gradually(P<0.05), while that in group C at different time points was not statistically significant. The expression of Cdh1 mRNA in group M increased significantly at the 1st d(P<0.05) and decreased at the 5th, 7th d compared with group C. Conclusion:The expression of Cdh1 mRNA in injured myeloid tissue decreased after SCI, which indicated that APC-Cdh1 might be involved in the path of pathophysiological mechanism after SCI. |
| Keywords:spinal cord injury cell cycle anaphase promoting complex gene expression |
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