| 宋国丽,苏海静,张小云.不同强度的静磁场对K562细胞的作用[J].中国康复医学杂志,2009,24(3):204~207 |
| 不同强度的静磁场对K562细胞的作用 点此下载全文 |
| 宋国丽 苏海静 张小云 |
| 深圳大学生命科学学院，深圳, 518060 |
| 基金项目： |
| DOI： |
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| 摘要： |
| 目的：研究不同强度的静磁场对体外培养的K562细胞的作用，探讨静磁场在恶性肿瘤治疗中的应用价值。方法：不同强度的磁场作用后，细胞计数、MTT法检测细胞生长增殖状态；瑞氏染色观察细胞形态变化；透射电镜观察磁场对细胞超微结构的影响；硝基四氮唑蓝还原实验检测细胞功能分化状况；流式细胞仪检测细胞周期和细胞表面抗原CD11b、CD14、CD71的变化； RT-PCR检测原癌基因c-myc表达。结果：静磁场能够抑制K562细胞增殖，高强度静磁场效应强于低强度静磁场；在静磁场作用下，细胞出现胞体缩小，核固缩，核质比下降等分化特征；同时细胞的NBT还原能力增强；磁场处理后，细胞表面CD11b、CD14的表达无明显变化（P>0.05）, 而CD71的表达增强（P<0.05），细胞周期阻断于G2/M期; 电镜图片显示，磁场作用后细胞细胞质浓缩，次级溶酶体数量增多，线粒体嵴出现断裂,核内染色质凝集成块。RT-PCR结果显示原癌基因c-myc的表达显著降低。结论：静磁场能够抑制K562细胞的增殖，诱导 K562细胞分化，抑制原癌基因表达，破坏K562细胞膜结构。 |
| 关键词：静磁场 白血病细胞 增殖 分化 |
| Effects of static magnetic fields of different intensities on K562 leukemia cells Download Fulltext |
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| College of Life Science, Shenzhen University, Shenzhen, 518060 |
| Fund Project: |
| Abstract: |
| Objective: To investigate the effects of static magnetic fields of different intensities on K562 leukemia cells, elucidate the potential application value of static magnetic field in cancer treatment. Method: K562 cells were exposed to static magnetic fields of different intensities. Cells proliferation were analyzed by cell count and methyl thiazolyl tetrazolium(MTT) assay. Changes of cell morphology and ultrastructure were detected under light microscopy and transmission electron microscopy(TEM). Nitroblue tetrazolium（NBT）-reducing test was used to assess leukemia cell differentiation. Cell cycle distribution and expression of cell membrane surface differentiation-related antigens (such as CD11b, CD14 and CD71) were determined by flow-cytometry. RT-PCR was used to evaluate expression of protooncogene c-myc. Result: Static magnetic field could inhibit the proliferation of K562 cells, the effect of magnetic field of higher intensity was more obvious than that of lower one. Static magnetic field could induce K562 cell differentiation, leading to nuclear condensation and nuclear-cytoplasm ratio descending. The NBT-reducing ability of K562 cells ascended in the groups exposed to static magnetic field. In addition, after treated with magnetic field, cell ratio of S phase increased, cell cycle arrested in G2/M phase, while the expressions of CD11b and CD14 did not obviously change(P>0.05) and expressions of CD71 increased significantly(P<0.05). Moreover,K562 cells treated with magnetic field presented protoplasm concentration, secondary lysosome increasing, mitochondrial cristae broken and chromatic agglutination. Expression of c-myc evidently decreased in magnetic field. Conclusion: Static magnetic field could inhibit proliferation of K562 cells, induce differentiation of K562 cells,inhibit the expression of protooncogene c-myc and destroy the membrane structure of K562 cell. |
| Keywords:static magnetic field leukemia cell proliferation differentiation |
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