| 朱雅斌,石翊飒,丁 力,马永丰.病理性疼痛对老年雄性大鼠海马细胞凋亡和胆碱乙酰转移酶的影响[J].中国康复医学杂志,2009,24(4):348~351 |
| 病理性疼痛对老年雄性大鼠海马细胞凋亡和胆碱乙酰转移酶的影响 点此下载全文 |
| 朱雅斌 石翊飒 丁 力 马永丰 |
| 兰州大学第二医院麻醉科，兰州，730030 |
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| 摘要： |
| 目的：研究病理性疼痛对老年雄性大鼠海马细胞凋亡和胆碱乙酰转移酶（CHAT）含量的影响。方法：60只老年雄性SD大鼠450—500g，随机分成3组，A组：单纯麻醉组；B组：假手术组；C组：模型组，结扎左侧坐骨神经各组再按术前1天、术后第1、7、14天4个时间点，分为4个亚组，每组5只。各组在热痛敏试验检测后，麻醉、灌注、开颅取脑，分别进行流式细胞仪检测、HE染色和CHAT免疫组化分析。结果：①术后第1天C组结扎侧热痛敏实验所致后肢回缩时间数值较基础值明显缩短，第7、14天热痛阈继续呈下降趋势，与A组相比，差异显著（P＜0.01）。②C组CA3区锥体细胞数目明显减少（P＜0.01）。③C组较A组和B组出现了更多的细胞凋亡（P＜0.01）。④C组CHAT阳性细胞数目比A组和B组明显减少（P＜0.01）。结论：病理性疼痛可以造成老年雄性大鼠热痛阈的明显下降和海马CA3区锥体细胞明显凋亡以及CHAT阳性细胞数目减少。 |
| 关键词：疼痛； 海马； 细胞凋亡 胆碱乙酰转移酶 |
| The impacts of pathological pain on the aged male rat hippocampus apoptosis and choline acetyltransferase Download Fulltext |
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| The Anaesthesia Department of the Second Hospital of Lanzhou University,Lanzhou，730030 |
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| Abstract: |
| Objective:To study the impacts of pathological pain on apoptosis of hippocampus and content of choline acetyltransferase(CHAT) in old male rats. Method:Sixty aged male SD rats 450—500g, were divided into 3 groups, A group: anesthesiaed group;B group: sham-operated group;C group: model group with left sciatic nerues ligated. Each group was divided into 4 sub-groups according to the time points: 1d before operation and the 1st d, 7th d, 14th d after operation, n=5. All groups were anesthesiaed, perfused, craniotomized for brain resecting. After heat-pain test, FCM, HE staining and CHAT immunization were detected. Result:①In group C the paw withdrawal latency(PWL) in heat-pain shortend apparently,and the heat-pain threshold continued on a downward trend at the 7th d and 14th d. There was significant difference compared with group A (P<0.01).②In group C the number of pyramidal cells of CA3 region reduced significantly（P＜0.01）. ③In group C, there were more apoptosis than that in group A and group B（P＜0.01）. ④The number of CHAT positive cells in group C was less significantly than that in group A and group B（P＜0.01）. Conclusion: The pathological pain could cause apparent decline of heat-pain threshold, significant apoptosis of the cells of CA3 region in hippocampus and reduction of CHAT positive cells in rats. |
| Keywords:pain hippocampus apoptosis choline acetyltransferase |
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