| 曹师承,孙黎光,叶立平,张 莹,王 玉.耐力运动对大鼠骨骼肌PKB磷酸化与表达的影响*[J].中国康复医学杂志,2009,24(5):416~418 |
| 耐力运动对大鼠骨骼肌PKB磷酸化与表达的影响* 点此下载全文 |
| 曹师承 孙黎光 叶立平 张 莹 王 玉 |
| 中国医科大学基础医学院生物化学与分子生物学教研室，沈阳，110001 |
| 基金项目：辽宁省教育厅高等学校科研基金资助项目(2004D244) |
| DOI： |
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| 摘要： |
| 目的：探讨耐力运动对大鼠骨骼肌蛋白激酶B（PKB）mRNA表达、蛋白总量（t-PKB）及磷酸化PKB（p-PKB)的影响。方法：SD大鼠随机分为对照组和运动组。运动组分为1 h/d、1.5 h/d组，运动7周，最后一次运动后24h和48h取材，分为1 h/d运动24h取材组、1 h/d运动48h取材组、1.5 h/d运动24h取材组、1.5 h/d运动48 h取材组。测定血清葡萄糖和胰岛素浓度；采用Western blot法检测t-PKB 和p-PKB水平；用RT-PCR法分析PKB mRNA的表达。结果 |
| 关键词：基因表达 蛋白激酶 骨骼肌 运动 |
| Effects of endurance exercises on PKB phosphorylation and gene expressions in skeletal muscles of rats Download Fulltext |
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| Department of Biochemistry and Molecular Biology, China Medical University, Shenyang,110001 |
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| Abstract: |
| Objective:To study the effects of exercises on PKB phosphorylation(p-PKB)，protein contents(t-PKB) and PKB mRNA gene expressions in skeletal muscles of rats. Method: All experimental rats were randomized into control group and exercises group. The rats of exercises group were trained for 7 weeks with different intensities and recovery periods of exercises. The changes in the expressions of t-PKB and p-PKB were determined with Western blotting. The expressions of PKB mRNA was determined with reverse transcription-polymerase chain reaction(RT-PCR). The serum levels of glucose and insulin were measured. Result: As compared with control, in exercises 1h/d and 1.5h/d groups, at the 24th h and 48th h after the last training p-PKB increased. After exercises 1.5h/d,at the 24th h and 48th h after the last training t-PKB increased. In exercises 1h/d and 1.5h/d groups, the 24th h after the last training, PKB mRNA expressions increased respectively. The serum levels of insulin in exercises rats decreased. Conclusion: Exercises could improve the PKB phosphorylation, protein contents and gene expressions, and elevate insulin sensitivity in skeletal muscles of rats. |
| Keywords:gene expression protein kinase skeletal muscle exercises |
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