| 陆雪松,姜亚军,秦洪云,王蓓蓓,林 强.大鼠脑出血后低氧诱导因子表达及中药干预的作用[J].中国康复医学杂志,2009,24(7):597~599 |
| 大鼠脑出血后低氧诱导因子表达及中药干预的作用 点此下载全文 |
| 陆雪松 姜亚军 秦洪云 王蓓蓓 林 强 |
| 东南大学附属中大医院康复科，南京，210009 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 1935 |
| 全文下载次数: 2895 |
| 摘要： |
| 目的：利用大鼠脑出血模型探讨脑出血期间脑组织中低氧诱导因子1α(HIF-1α)表达的时程和分布特征及中药干预对脑出血后HIF-1α表达的影响。方法： 39只体重250—300g的健康成年雄性SD大鼠，采用Nath改良法建立脑出血模型，随机分为脑出血组、中药（脑血通）干预组，另设生理盐水和正常组作为对照。分别于造模后第6h、24h、72h、第7天，利用Bederson等的3级标准评定动物的神经系统功能，然后分别处死取脑用免疫组织化学法检测HIF-1α的表达。结果：正常脑组织和生理盐水组无HIF-1α的表达。脑出血后第6小时同侧基底核血肿区周围出现HIF-1α表达增加，至第72小时达高峰，第7天明显下降。与单纯脑出血组比较，中药干预后可以使上述各时间点HIF-1α表达较脑出血组减少，在第24h、72h、7d有显著性意义(P<0.01)，神经功能障碍体征改善在第72h、第5天时间点有显著性意义（P<0.05）。结论：脑出血后血肿周围HIF-1α的表达明显上调，并可能参与了脑出血后血肿周围神经细胞的继发性损伤过程；中药具有神经保护作用，能有效地抑制脑出血后HIF-1α表达。 |
| 关键词：脑出血 低氧诱导因子-1?琢 凝血酶 |
| The expression of hypoxia inducible factor-1α and its regulation with traditional Chinese medicine in rats models of experimental intracerebral hemorrhage Download Fulltext |
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| Dept. of Rehabilitation, Zhongda Hospital, Southeast University, Nanjing，210009 |
| Fund Project: |
| Abstract: |
| Objective: To investigate the changes of expressions of hypoxia-inducible factor-1α (HIF-1α) and its distribution in brain tissue in rats models of experimental intracerebral hemorrhage(ICH), and the possible effects of traditional Chinese medicine on the expressions of HIF-1α. Method: The rats′ model was established by Nath modified method. Thirty-nine male Sprague-Dawley rats (weight 250—300g) were randomly divided into intracerebral hemorrhage group and intracerebral hemorrhage treated with traditional Chinese medicine（naoxuetong）group; saline group and normal group were negative controls. At the 6th, 24th,72nd h and the 7th d after operation, the rat′s neurological functions were assessed with Bederson′s grade. Then rats were sacrificed respestively, and the brain tissues were taken out. Immunohistochemistry analyses of expressions of HIF-1α were observed. Result: HIF-1α was undetectable in brain tissues at normal group and saline group. However, the positive HIF-1α expressions in neurons were detected in ipsilateral basal ganglia around the hematoma region in intracerebral hemorrhage group, with up-regulation of HIF-1α expressions occurred as early as at the 6th h, peaked at the 72nd h, and then declined at the 7th d. Compared with intracerebral hemorrhage group HIF-1α expressions down-regulated significantly in intracerebral hemorrhage treated with traditional Chinese medicine group at the 24th h、72nd h、7th d(P<0.01)，and neurological functions improved significantly at the 72nd h、5th d(P<0.05). Conclusion: The expressions of HIF-1α up-regulated after experimental intracerebral hemorrhage around the hematowa in rats, which may contribute to the secondary cerebral injury process surrounding hematoma；the protective effects of traditional Chinese medicine may be related with inhibiting the expressions of HIF-1α. |
| Keywords:intracerebral hemorrhage hypoxia-inducible factor-1α thrombin |
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