| 孔立红,刘胜洪,毛娟娟,周 华,杜艳军.电针对脑缺血大鼠NF-κB及TNF-?琢表达的影响*[J].中国康复医学杂志,2009,24(8):711~714 |
| 电针对脑缺血大鼠NF-κB及TNF-?琢表达的影响* 点此下载全文 |
| 孔立红 刘胜洪 毛娟娟 周 华 杜艳军 |
| 湖北中医学院，武汉，430061 |
| 基金项目：湖北省自然科学基金课题（2003ABA154） |
| DOI： |
| 摘要点击次数: 2222 |
| 全文下载次数: 2029 |
| 摘要： |
| 目的：探讨核转录因子-?资B (NF-?资B)活性和肿瘤坏死因子（TNF-α）在脑缺血再灌注大鼠海马表达变化的规律和电针干预对其影响。方法：将SD大鼠随机分为正常组、假手术组、模型组、电针治疗组。采用改良线栓法制备局灶型脑缺血（MCAO）再灌模型，电针“大椎”、双侧“内关”穴。运用免疫组化检测法观察海马组织NF-κB-p65蛋白的表达及核转位；用放射免疫法测定各组大鼠海马组织中TNF-α含量的变化。结果：模型各组大鼠缺血侧海马CA1区NF-κB-p65蛋白表达和海马组织TNF-α含量显著增高（P＜0.01）。与模型各组大鼠相比，电针治疗组缺血侧海马组织CA1区NF-κB-p65蛋白表达和TNF-α含量均显著降低。结论：TNF-α的表达上调后可活化NF-κB，参与脑损伤后继发性炎症反应过程，电针能抑制其活化可以减轻脑缺血再灌注时的炎症反应，发挥神经保护作用。 |
| 关键词：电针；脑缺血再灌注；海马；核转录因子 肿瘤坏死因子 |
| Effects of acupuncture on the expression and content of nuclear factor-κB and tumor necrosis factor-?琢 in rat brain after cerebral ischemia/reperfusion Download Fulltext |
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| Hubei College of Traditional Chinese Medicine, Wuhan，430061 |
| Fund Project: |
| Abstract: |
| Objective:To investigate effects of acupuncture on the expression and content of nuclear factor-κB(NF-κB) and tumor necrosis factor-α(TNF-α) in hippocampus of rat brain after cerebral ischemia/reperfusion. Method: SD rats were randomly divided into normal group, sham operation group,model group, and electroacupuncture group. The rat′s middle cerebral artery was blocked to establish cerebral ischemia（MCAO）/reperfusion model with suturing MCA.“Dazhui”(BU14),“Neiguan”(PC6) of both side were treated with electroacupuncture. The expression and content of nuclear factor-κB in hippocampus were detected with immunohistochemistry and the content of TNF-α in hippocampus were detected by means of radio-immunoassay(RIA). Result:The expression of NF-κB-p65 in ischemic hippocampi CA1 and content of TNF-α in hippocampus of rats in model group were significantly higher than those in electroacupuncture group（P<0.01,<0.05）. Conclusion: NF-κB and TNF-α may play an essential role in injury-induced inflammatory response that leads to secondary insults after brain injury.Electroacupuncture may inhibit the expression of NF-κB in hippocampus of rat brain after cerebral ischemia/reperfusion, reduce the inflammatory response and block it migrating into nucleus. Electroacupuncture can provide the protective effects on neuronal cells after cerebral ischemia/reperfusion in rats. |
| Keywords:electroacupuncture cerebral ischemia reperfusion hippocampus nuclear factor-κB tumor necrosis factor-α |
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