| 徐义明,白跃宏,俞 红.腰痛患者肌卫星细胞生物学特性及其鉴定[J].中国康复医学杂志,2009,24(8):734~736 |
| 腰痛患者肌卫星细胞生物学特性及其鉴定 点此下载全文 |
| 徐义明 白跃宏 俞 红 |
| 上海交通大学附属第六人民医院康复医学科，上海市宜山路600号, 200233 |
| 基金项目： |
| DOI： |
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| 摘要： |
| 目的：观察腰痛患者肌卫星细胞体外培养的生物学特性，探讨其在慢性腰痛中肌纤维损伤的修复作用。 方法：取腰骶部慢性骨筋膜间隔综合征所致腰痛患者活体竖脊肌组织块，采用I型胶原酶和胰蛋白酶两步消化法及差速贴壁法纯化肌卫星细胞，进行原代培养及传代培养，观察细胞形态及其传代能力，绘制生长曲线，结蛋白免疫组化染色鉴定肌卫星细胞。 结果： 纯化后肌卫星细胞生长良好，传代培养后增殖速度较原代培养快，6代以前形态较规则，8代以后逐渐老化，结蛋白鉴定呈阳性。 结论：慢性腰痛患者肌卫星细胞体外培养形态与正常人基本相同，但传代能力减弱，可能与修复受损肌纤维有关。 |
| 关键词：卫星细胞 腰痛 细胞培养 |
| Biological characteristics and identification of skeletal muscle satellite cells in patients with low back pain Download Fulltext |
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| Department of Rehabilitation， Shanghai Sixth People Hospital，Shanghai Jiaotong University School of Medicine, Shanghai,200233 |
| Fund Project: |
| Abstract: |
| Objective：To observe biological characteristics of skeletal muscle satellite cells in patients with low back pain in vitro and to discuss its contribution to repairing damaged muscle fibers. Method：The human erector spinae tissues of patients with low back pain induced by chronic compartment syndrome were obtained through surgery. Muscle satellite cells were purified by two-step digest method of collagenase-I and trypsin and the method of different-speed adherence were subject to primary as well as passage culture in vitro. Morphological characteristics，growth curve were observed to evaluate the proliferation, differentiation and passage ability of muscle satellite cells. The cells were identified with cellular immunochemical stain. Result：The purified cells showed strong proliferative ability which was stronger in passage culture than primary culture. The morphous of cells were regular before the sixth generation, but commenced to be aged since the eighth generation. Endochylemas were positive by cellular immunochemical stain with desmin. Conclusion：Morphological characteristics of muscle satellite cells in patients with low back pain induced by chronic compartment syndrome were similar with health people, while their passage abilities were weak, which may result in skeletal muscle satellite cells lose repairing of the damaged muscle fibers. |
| Keywords:satellite cell low back pain cell culture |
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