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王文婷,周谋望,陈庆山,杨立元,杨 磊,黄红拾.脊髓损伤大鼠脊髓及泌尿生殖道caspase-3和nNOS表达的改变[J].中国康复医学杂志,2009,24(9):
脊髓损伤大鼠脊髓及泌尿生殖道caspase-3和nNOS表达的改变    点此下载全文
王文婷  周谋望  陈庆山  杨立元  杨 磊  黄红拾
北京大学第三医院康复医学中心,100191
基金项目:国家自然科学基金资助项目(30672096)
DOI:
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摘要:
      目的:观察SD大鼠脊髓损伤后脊髓及泌尿生殖道凋亡促进因子半胱氨酸天冬氨酸蛋白酶-3(caspase-3)和一氧化氮合成酶(nNOS)表达的改变。方法: 成年雄性SD大鼠16只,随机分为对照组(n=8)和脊髓损伤组(n=8);脊髓损伤组采用改良的重物坠落法(Allen法)制作大鼠T9-T10脊髓节段不完全损伤模型。分别行caspase-3免疫组化及nNOS免疫组化染色,用半定量方法评价正常及脊髓损伤大鼠脊髓、膀胱壁及阴茎组织表达caspase-3和nNOS的改变情况。结果:脊髓损伤后大鼠脊髓内nNOS和caspase-3均表达增强(P<0.001),而膀胱壁及阴茎组织内caspase-3表达增强(P<0.001),nNOS表达减弱(P<0.001)。结论:脊髓损伤大鼠脊髓及泌尿生殖道表达caspase-3和nNOS发生了改变,但并非同步,机制各有不同。
关键词:脊髓损伤  脊髓  泌尿生殖系统  半胱氨酸天冬氨酸蛋白酶-3  一氧化氮合成酶
Changes of the expressions of caspase-3 and nNOS in spinal cord and urogenital system of rats with spinal cord injury    Download Fulltext
Rehabilitation Center of Peking University Third Hospital,Beijing,100191
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Abstract:
      Objective: To research the changes of the expressions of cysteine asparate protease-3(caspase-3) and nitric oxide synthase(nNOS) in spinal cord and urogenital system of SD rats with spinal cord injury. Method: Sixteen male SD rats of clean grade, age 6 weeks, were selected and randomized into spinal cord injury group (n=8) and control group (n=8). Rats in spinal cord injury group were crashed at T9-T10 vertebra to cause spinal cord incomplete injury models using modified Allen′s method, while only laminectomy without crash was administered in control group. The injured spinal cord segment, bladder and the middle part of penis were taken for analysis, and contrasted with control rats. Caspase-3 and nNOS positive cells were marked with immunohistochemistry technique, and the results were evaluated with semiquantitative analysis. Result: After injuries, the expressions of caspase-3 and nNOS increased significantly in spinal cord (P<0.001); in bladder wall and penis, caspase-3 expressed much more(P<0.001), but nNOS positive neurons and fibers decreased dramatically(P<0.001). Conclusion: There were great changes in expressions of caspase-3 and nNOS in spinal cord, bladder and penis of rats after spinal cord injury with different mechanism.
Keywords:spinal cord injury  spinal cord  urogenital system  cysteine asparate protease-3  nitric oxide synthase
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