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何 梅,覃 霞,陈鹏慧,杨宗城.烧伤后慢性应激对大鼠海马微管相关蛋白-2和胶质纤维酸性蛋白表达的影响[J].中国康复医学杂志,2009,24(9):
烧伤后慢性应激对大鼠海马微管相关蛋白-2和胶质纤维酸性蛋白表达的影响    点此下载全文
何 梅  覃 霞  陈鹏慧  杨宗城
第三军医大学西南医院烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆,400038
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DOI:
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摘要:
      目的:探讨烧伤后慢性应激大鼠海马神经元微管相关蛋白-2(MAP-2)和神经胶质细胞胶质纤维酸性蛋白(GFAP)表达的变化。方法:采用30%深Ⅱ度烫伤、孤养及慢性中度不定期应激联合建立烧伤后并发抑郁模型,采用旷场实验观察烧伤后并发抑郁大鼠行为反应,电镜和免疫组化观察不同区域海马MAP-2和GFAP的表达及神经元和神经胶质细胞超微结构的变化。结果:烧伤后并发抑郁大鼠活动潜伏期明显延长,行为反应明显降低(P<0.01)。烧伤后并发抑郁、烧伤、抑郁及正常组之间MAP-2和GFAP在CA1区变化不明显;烧伤后并发抑郁大鼠在CA3区MAP-2和GFAP阳性细胞计数和累积光密度(89.58±15.18,69162.63±7905.36;57.33±6.50,37918.42±2060.98)明显低于正常组(108.80±18.45,84607.07±13602.34;90.50±5.98,55616.67±3776.54;P<0.05);在齿状回烧伤并发抑郁大鼠MAP-2和GFAP阳性细胞计数和累积光密度(261.00±35.37,32199.93±1344.15;73.42±7.32,68465.21±15571.64)与正常组相比(293.00±24.47,40299.77±1155.97;113.10±6.72,108416.10±5128.89)也明显降低(P<0.05)。电镜可见烧伤并发抑郁组神经元和神经胶质细胞胞浆肿胀,有的线粒体髓鞘样改变,线粒体肿胀、空泡化,内质网扩张等改变。结论:海马神经元和神经胶质细胞结构改变可能是烧伤后抑郁发生的重要病理生理基础。
关键词:烧伤  慢性应激  微管相关蛋白-2  胶质纤维酸性蛋白
The influence of chronic stress after burn on the expressions of MAP-2 and GFAP in hippocampus of rats    Download Fulltext
Burn Department of Southwest Hospital, Third Military Medical University, Chongqing,400038
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Abstract:
      Objective:To study the expressions of microtubule associated protein-2(MAP-2) and glial fibrillary acidic protein(GFAP) in hippocampus of rats with depression after burn. Method:The depression after burn model of rats was established by 30% deep partial thickness burn combined with chronic unpredicted mild stress and separation feed. The behavior reaction of depression after burn on rats were investigated. The expressions of MAP-2 and GFAP and ultrastructures of neurons and astrocytes in hippocampus were analyzed. Result: Compared with controls, there were significomt influences of depression after burn on the open-field behavior of rats: the latency prolonged and the behavior reaction reduced(P<0.01). The number and accumulate photodensity of MAP-2 and GFAP in CA3 area of depression after burn rats decreased significantly(89.58±15.18,69162.63±7905.36;57.33±6.50,37918.42±2060.98) than that in control group(108.80±18.45,84607.07±13602.34;90.50±5.98,55616.67±3776.54), P<0.05. The number and accumulate photodensity of MAP-2 and GFAP in dentate gyrus(DG) of depression after burn rats also decreased significantly(261.00±35.37, 32199.93±1344.15; 73.42±7.32, 68465.21±15571.64) than that in control group(293.00±24.47, 40299.77±1155.97; 113.10±6.72, 108416.10±5128.89), P<0.05. The observation under electron microscope showed swelling and vacuolization of mitochondrion,myelin sheath irregular changes of neurons and astrocytes in hippocampus of rats with depression after burn. Conclusion: The changes of ultrastructure of neurons and astrocytes in hippocampus may be the important pathophysiological basis of depression after burn in rats.
Keywords:burn  chronic scress  microtubule associated protein-2  glial fibrillary acidic protein
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