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韩振翔,魏江磊,祁丽丽,李妙龄.生地预处理在减轻全脑缺血再灌注大鼠海马神经元损伤中的作用[J].中国康复医学杂志,2009,24(9):
生地预处理在减轻全脑缺血再灌注大鼠海马神经元损伤中的作用    点此下载全文
韩振翔  魏江磊  祁丽丽  李妙龄
上海中医药大学附属曙光医院中风专科,上海,200021
基金项目:上海市科委登山项目(课题编号:06JC14027)
DOI:
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全文下载次数: 1957
摘要:
      目的:观察比较中药生地预处理与缺血预处理减轻全脑缺血再灌注大鼠海马神经元损伤的作用。方法:实验选用24只雄性SD大鼠,随机将24只大鼠分为假手术组、缺血预处理组、生地预处理组、脑缺血再灌注组,采用热凝椎动脉,钳夹双侧颈总动脉建立全脑缺血模型,缺血预处理组预缺血3min,3d后给予缺血10min,再灌注24h后处死。假手术组暴露双侧颈总动脉不夹闭。缺血再灌注组,夹闭双侧大脑颈总动脉10min,再灌注24h后处死。生地预处理组,灌胃2周后,作缺血再灌注处理,运用HE染色光镜下观察存活海马神经元细胞数,电镜下观察海马神经元超微形态的改变。结果:生地预处理存活神经元数与缺血预处理存活神经元数比较,P>0.05,两者与缺血再灌注组比较,P<0.01。假手术组可见海马神经元细胞形态正常,脑缺血预处理组和药物预处理组海马CA1区神经元损伤明显减轻,只有少量细胞有轻度水肿,未见坏死。而脑缺血再灌注组神经元变性严重。结论:生地预处理对随后的脑梗死有明显的保护作用,能诱导缺血耐受的产生。
关键词:脑缺血  缺血预处理  生地预处理  海马神经元
An experimental study on shengdi pretreatment effect on the cerebral ischemia-reperfusion injury in rats    Download Fulltext
Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai,200021
Fund Project:
Abstract:
      Objective:To investigate the shengdi pretreatment effect and the ischemia preconditioning effect on the changes of neuron structure of hippocampal region in rats with global cerebral ischemia-reperfusion. Method:Twenty-four male SD rats(weight 180-220g)were randomly divided into 4 groups: sham operation group(n=5); ischemia-reperfusion group(n=5); ischemia preconditioning group (n=5); shengdi pretreatment group (n=5). Global cerebral ischemia and reperfusion model were established with Pulsinelli′s method. The amount and ultrastructure of neurons of hippocampus were observed under light microscope and electron-microscope. Result:Compared with ischemia-reperfusion group,the number of survival neurons of hippocampal CAl region increased significantly (P<0.01), and the injury of neurons lightened significantly and no necrosis in shengdi pretreatment group and ischemia preconditioning group. Compared shengdi pretreatment group with ischemia preconditioning group,the number of survival neuron had no distinct significance in hippocampal CA1 region(P>0.05). Conclusion:Shengdi pretreatment can obviously increase brain tissue tolerance to secondary severe infarction and present apparent protection effect.
Keywords:cerebral ischemia  ischemic preconditioning  shengdi pretreatment  hippocampus
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