| 李 花,刘旺华,周小青,何 倩.活血通络组方对大鼠脑缺血再灌注损伤后神经功能恢复及其抗凋亡机制研究[J].中国康复医学杂志,2009,24(10):880~883 |
| 活血通络组方对大鼠脑缺血再灌注损伤后神经功能恢复及其抗凋亡机制研究 点此下载全文 |
| 李 花 刘旺华 周小青 何 倩 |
| 湖南中医药大学基础医学院临床基础学科，湖南长沙，410007 |
| 基金项目：湖南省自然科学基金项目（07JJ6056）；湖南省教育厅科研项目（07C475） |
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| 摘要： |
| 目的：探讨活血通络组方对大鼠脑缺血再灌注损伤后神经功能恢复及其抗凋亡机制研究。方法：大脑中动脉闭塞再通法建立脑缺血再灌注模型，采用神经行为学评分评价神经功能的恢复，跳台法测试大鼠学习和记忆成绩，原位末端标记法检测凋亡神经元，用免疫组化法检测Fas相关死亡结构域蛋白（FADD）、凋亡蛋白酶（caspase-8、caspase-3）阳性细胞数。结果：与模型组比较，活血通络组的学习和记忆成绩均优于模型组，神经功能损伤评分亦有明显降低，神经元凋亡指数（AI）、FADD、caspase-8、caspase-3阳性细胞数均显著减少。结论：FADD、caspase-8、caspase-3在脑缺血再灌注后细胞凋亡过程中发挥重要作用，活血通络组方能减轻脑缺血再灌注后神经功能损伤，其机制可能与抑制细胞凋亡死亡受体通路有关。 |
| 关键词：脑缺血再灌注 神经功能 Fas相关死亡结构域蛋白 凋亡蛋白酶 活血通络组方 |
| A study on the effects of huoxuetongluo recipe on neural function recovery after cerebral ischemia reperfusion injury in rats and its mechanism of anti-apoptosis Download Fulltext |
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| The Basic Subject of Clinic of TCM, School of Preclinical Medicine, Hunan University of Traditional Chinese Medicine, Changsha,410007 |
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| Abstract: |
| Objective: To study the effects of huoxuetongluo recipe on neural function recovery after cerebral ischemia reperfusion injury in rats and its mechanism of anti-apoptosis. Method: The model of cerebral ischemia reperfusion（I/R） was induced by middle cerebral artery occlusion(MCAO) and recanalization. Terminal deoxyribo-nucleotide transferase-mediated dUTP-biotin nick end labeling(TUNEL) method was used to detect the apoptotic neurocytes. The positive neurons of Fas associated with death domain protein(FADD)、caspase-8 and caspase-3 were detected by immunohistochemistry. Result: Compared with model group, the apoptotic index(AI) and the positive neurons of FADD, caspase-8, caspase-3 decreased markedly in huoxuetongluo group, the learning and memory abilities also increased, the scores of neural function impairment decreased significantly(P<0.01—0.05). Conclusion: FADD, caspase-8 and caspase-3 play important roles in the apoptosis after cerebral I/R injury. Huoxuetongluo recipe could improve the neural function, and the mechanism maybe concern with suppressing the pathway of apoptosis death receptors. |
| Keywords:cerebral ischemia reperfusion neural function Fas-associated with death domain protein caspase huoxuetongluo recipe |
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