| 姜大朋,李昭铸,蒋志涛,刘君阳,李天竹,王 娜.肝细胞生长因子阻抑TGF-β1诱导大鼠韧带成纤维细胞?琢-SMA过表达及其机制[J].中国康复医学杂志,2009,24(10):906~909 |
| 肝细胞生长因子阻抑TGF-β1诱导大鼠韧带成纤维细胞?琢-SMA过表达及其机制 点此下载全文 |
| 姜大朋 李昭铸 蒋志涛 刘君阳 李天竹 王 娜 |
| 哈尔滨医科大学附属第二医院小儿外科，150086 |
| 基金项目：黑龙江省自然科学基金项目（D200618）；国家自然科学基金青年基金项目（30901516） |
| DOI： |
| 摘要点击次数: 2297 |
| 全文下载次数: 2062 |
| 摘要： |
| 目的：研究肝细胞生长因子（HGF）能否阻抑TGF-β1诱导的大鼠内侧副韧带（MCL）成纤维细胞?琢-SMA过表达及其可能涉及的信号传导通路。方法：采用组织块培养法培养大鼠MCL成纤维细胞，培养液中加入TGF-β1(5ng/ml)及HGF (10—40 ng/ml)。培养72h后，用RT-PCR检测各组?琢-SMA mRNA及Smad3 mRNA的变化；细胞免疫组化检测?琢-SMA蛋白的表达。结果：TGF-β1能显著诱导?琢-SMA及Smad3的表达(P<0.01)，而HGF则可以有效地阻抑其表达，其效应呈剂量依赖性(P<0.05)。结论：HGF可以通过下调Smad3的表达来阻抑TGF-β1诱导的?琢-SMA过表达。这为利用HGF预防和治疗MCL损伤后瘢痕及纤维化在细胞和分子水平提供了依据。 |
| 关键词：肝细胞生长因子 转化生长因子-β 内侧副韧带 成纤维细胞 ?琢-平滑肌肌动蛋白 |
| Hepatocyte growth factor suppresses the overproduction of α-SMA induced by TGF-β1 in rat medial collateral ligament fibroblasts Download Fulltext |
|
| Department of Pediatric Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, 150086 |
| Fund Project: |
| Abstract: |
| Objective：To examine the effectiveness of HGF in blocking TGF-β1-induced ?琢-SMA production in rat medial collateral ligament (MCL) fibroblasts. Method：Fibroblasts were obtained from rat MCL. Cell culture was supplemented with 5ng/ml of TGF-β1 along with increasing doses of HGF (10-40 ng/ml). After 72 hours incubation, the productions of ?琢-SMA and Smad3 mRNA were assayed by RT-PCR. Expression of ?琢-SMA protein was assessed by immunostaining. Result：Treatment with TGF-β1 significantly stimulated ?琢-SMA and Smad3 mRNA production in MCL fibroblasts (P<0.01). Remarkably, the addition of HGF reduced productions of all components induced by TGF-β1 in a dose-dependent manner (P<0.05). Conclusion：HGF antagonizes TGF-β1 induced ?琢-SMA production in MCL fibroblasts by down regulating Smad3. The findings provide a cellular and molecular basis for HGF′s acting as a therapeutic agent for MCL scar and fibrosis formation. |
| Keywords:hepatocyte growth factor transforming growth factor-β medial collateral ligament fibroblast ?琢-smooth muscle actin |
|
| 查看全文 查看/发表评论 |
