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李志宏,周振华,谢菊英,唐吉平.坐骨神经和股神经离断、后肢固定对大鼠降钙素基因相关肽水平及骨密度的影响[J].中国康复医学杂志,2009,24(12):1112~1114
坐骨神经和股神经离断、后肢固定对大鼠降钙素基因相关肽水平及骨密度的影响    点此下载全文
李志宏  周振华  谢菊英  唐吉平
湖南城市学院,湖南益阳,413000
基金项目:
DOI:
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摘要:
      目的:明确失神经、固定对大鼠降钙素基因相关肽水平及骨密度的影响,探索失神经和固定引起的骨质疏松的可能机制。方法:10周龄SD雄性大鼠96只,随机分为3组,每组4个时间点,每时间点8只。失神经支配组接受双侧坐骨神经和股神经切断术,固定组大鼠双侧后肢利用石膏管型固定,对照组大鼠接受假手术。主要观察指标:造模后第1、10、30、60天各组大鼠胫骨骨密度和降钙素基因相关肽的变化及降钙素基因相关肽水平与骨密度水平相关性。结果:①降钙素基因相关肽:造模后第10、30、60天失神经支配组大鼠胫骨降钙素基因相关肽的水平低于对照组(P< 0.05—0.01);而固定组仅造模后第30天与对照组之间存在显著性差异(P<0.05)。造模后第10、60天固定组高于失神经支配组(P<0.05—0.01)。②骨密度:与对照组相比,失神经支配组、固定组造模后第30、60天骨密度降低(P<0.05—0.01);失神经支配组和固定组造模第30天骨密度开始下降,60d后下降明显,组内比较差异有显著性意义(P<0.05—0.01)。③失神经后降钙素基因相关肽与骨密度水平变化高度相关(P<0.05);而固定后降钙素基因相关肽与骨密度水平变化无明显相关性(P>0.05)。结论:失神经可导致骨组织降钙素基因相关肽水平下降,降钙素基因相关肽水平下降是失神经性骨质疏松的可能机制之一;固定引起的骨质疏松的发生与降钙素基因相关肽的变化无关。
关键词:失神经  固定  降钙素基因相关肽  骨密度
Effects on the level of calcitonin gene-related peptide and bone mineral density after sciatic and femoral denervation or hinder limb immobilization    Download Fulltext
Hunan City University, Yiyang, 413000
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Abstract:
      Objective: To explicit the effects on the level of calcitonin gene-related peptide(CGRP) and bone mineral density(BMD) after denervation and immobilization, and explore the possible mechanism of osteoporosis caused by denervation and immobilization. Method: Ninety-six male SD rats were randomly divided into 3 groups: denervated group, immobilized group and control group, with 4 time points in each group and 8 rats in each time point. Denervated group received the bilateral sciatic nerve and femoral nerve cutting off. Immobilized group received hinder limbs immobilizing with tube plaster. Control group only received sham operation. Main outcomes were measured: content changes of CGRP and BMD as well as relevance between CGRP and BMD at the 1st d, 10th d, 30th d and 60th d after model preparation. Result: ①CGRP: The expression of CGRP was lower in denervated group at the 10th d, 30th d and 60th d after model preparation (P<0.05—0.01), with statistical difference between immobilized group and control group at the 30th d after immobilization (P<0.05). The expression of CGRP was higher in immobilized group than that in denervated group at the 10th d and 60th d after model preparation (P<0.05—0.01). ②BMD: Compared with control group, content of BMD in immobilized group and denervated group decreased at the 30th d and 60th d after model preparation (P<0.05—0.01), beginning decrease from the 30th d, and obviously decreasing at the 60th d in immobilized group and denervated group. There were significant differences in intra-group comparison (P<0.05—0.01). ③CGRP was highly related to BMD after denervation (P<0.05). However, there was no obvious relation between CGRP and BMD after immobilization(P>0.05). Conclusion: Denervation can decrease the level of CGRP , and CGRP is one of possible mechanisms of osteoporosis caused by denervation. It is irrelevant between CGRP and osteoporosis caused by immobilization.
Keywords:denervation  immobilization  calcitonin gene-related peptide  bone mineral density
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