| 李 哲,方征宇,黄晓琳.不同剂量U0126抑制低频磁刺激引起星形胶质细胞迁移的实验研究[J].中国康复医学杂志,2010,25(3):195~199 |
| 不同剂量U0126抑制低频磁刺激引起星形胶质细胞迁移的实验研究 点此下载全文 |
| 李 哲 方征宇 黄晓琳 |
| 华中科技大学附属同济医院，武汉，430030 |
| 基金项目：国家自然科学基金资助项目（30801220） |
| DOI： |
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| 摘要
目的：研究不同剂量U0126对低频磁刺激促进星形胶质细胞迁移作用的影响，以选择合适的阻滞剂量。
方法：24只SD大鼠按U0126剂量被随机分为对照组（0mg/kg U0126，n=6）、低剂量组（0.1mg/kg U0126，n=6）、中剂量组（0.2mg/kg U0126，n=6）、高剂量组（0.4mg/kg U0126，n=6），四组的刺激参数都为频率1Hz，强度1.52T，刺激量为30脉冲；均采用溴已锭注射入脊髓左侧背索复制局灶性的脊髓损伤模型。刺激后第14天，大鼠被处死，采用图像分析系统观察GFAP、MAP-2和ERK1/2的表达及脊髓损伤空洞体积的变化。
结果：随着U0126剂量的增加，在第14天时空洞的体积逐渐增大，GFAP、ERK1/2的阳性表达逐渐减弱，与对照组相比具有显著性差异(P<0.05)，U0126中剂量组和高剂量组作用则无明显差异（P>0.05）；病灶区域MAP-2均呈阴性表达。
结论：不同剂量的U0126可翻转磁刺激引起的星形胶质细胞的迁移，0.2mg/kg是较合适的剂量。 |
| 关键词：低频 磁刺激 星形胶质细胞 迁移 |
| The effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration Download Fulltext |
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| Department of Rehabilitation, Tongji Hospital, Huazhong University of Science and Technology，Wuhan，430030 |
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| Abstract: |
| Abstract
Objective：To investigate the effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration and to select suitable dose of U0126.
Method：Twenty-four adult healthy SD rats were selected to receive injection of 0.5ml of 1% ethidium bromide (EB) in PBS into the dorsal spinal cord funiculus on the left side at T10-11 level to make located spinal cord injury models and randomly divided into four groups.The four groups were exposed to magnetic stimulation(1Hz，1.52T，30pulses) at the following dose respectively: 0mg/kg U0126(control group），0.1mg/kg U0126(low-dose group），0.2mg/kg U0126(middle-dose group），0.4mg/kg U0126(high-dose group）. On the day 14 after stimulation, the rats were sacrificed and the expressions of glial fibrillary acidic protein(GFAP), microtubule associated protein-2 (MAP-2), extracellular signal-regulated kinase1/2 (ERK1/2) and the volume of holes were detected with immunohistochemistry. Quantitative analysis of the expressions of GFAP,MAP-2 and ERK1/2 were performed with the image analysis system.
Result：With the increase of U0126 dose,the volume of hole increased on day 14(P<0.05). In the lesion area,the expressions of GFAP and ERK1/2 could be found,while MAP-2 could not. Significant differences were revealed in the expressions of GFAP、ERK1/2 among the four groups, it was significantly lower in U0126 groups than that in control group(P<0.05). while the middle-dose group had similar effect with the high-dose group（P>0.05）.
Conclusion：U0126 of different doses all could resupinate astrocyte migrations which were coused by low frequency magnetic stimulation, and 0.2mg/kg was the suitable dose. |
| Keywords:low frequency magnetic stimulation astrocyte migration |
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