| 侯伊玲,薄 海,刘子泉,吴振军,姜铁民,李 贺.运动训练对心肌梗死后心室重构及MMP-2，MMP-9表达的影响[J].中国康复医学杂志,2010,25(3):200~204 |
| 运动训练对心肌梗死后心室重构及MMP-2，MMP-9表达的影响 点此下载全文 |
| 侯伊玲 薄 海 刘子泉 吴振军 姜铁民 李 贺 |
| 武警医学院附属医院，武警部队心血管病研究所，天津，300162 |
| 基金项目：天津市科委自然科学基金重点项目资助（05YFJZJC01602） |
| DOI： |
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| 摘要： |
| 摘要
目的：观察大鼠心肌梗死（MI）后早期运动训练对左心室（LV）重构、心室功能、心肌间质胶原、基质金属蛋白酶（MMPs）的影响。
方法：SD大鼠随机分为假手术组（Sham组）、MI对照组（AMI-Sed组）和MI+运动训练组（AMI-Ex组），结扎前降支建立MI模型。手术后1周Ex组进行8周跑台训练（运动强度相当于45%VO2max），术后第9周测量各组大鼠左心室重量、血液动力学参数，处死大鼠，用Masson染色检测左心室非梗死区心肌胶原容积分数（CVF），RT-PCR检测Ⅰ、Ⅲ型胶原纤维mRNA表达，Western blot 检测非梗死区心肌基质金属蛋白酶-2（MMP-2）及基质金属蛋白酶-9（MMP-9）蛋白表达。
结果：与Sham组比较，AMI-Sed、AMI-Ex组左心室重量指数（LVWI）和左心室长轴径（LVLA）均显著增加（P<0.05），运动训练对LVWI和LVLA无明显影响；与AMI-Ex组比较，AMI-Sed组RVWI、肺含水率显著增高，左心室压峰值（LVSP）和左心室压力变化最大值（+dp/dt max）显著降低，左心室压力负最大值（LVEDP）显著升高（P均<0.05）；与AMI-Sed组比较，AMI-Ex组大鼠左心室非梗死区CVF降低，Ⅰ、Ⅲ型胶原纤维mRNA表达量均降低（P<0.05），以Ⅰ型胶原纤维表达降低为著；与Sham组比较，AMI-Sed、AMI-Ex组MMP-2表达均显著升高（P<0.05），MMP-9无显著变化；AMI-Ex组MMP-2蛋白表达显著低于AMI-Sed组（P<0.05）。
结论：AMI后早期运动训练可抑制非梗死区心肌纤维化、改善左心室重构及左心室功能，机制可能与抑制胶原纤维合成有关，MMP-2表达变化对此可能发挥一定作用。 |
| 关键词：心肌梗死 心室重塑 运动 胶原 基质金属蛋白酶-2 基质金属蛋白酶-9 |
| Effects of exercises training on myocardial remodeling and MMP-2 and MMP-9 expressions in post myocardial infarction rats Download Fulltext |
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| Pingjin Hospital, Medical College of CAPF, Institute of Cardiovascular Disease of CAPF, Tianjin, 300162 |
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| Abstract: |
| Abstract
Objective： To investigate the role of exercises training(ET) on left ventricular(LV) remodeling and dysfunction in post-ischemic failing rats by ligation of the left descending coronary artery.
Method: Adult SD male rats were randomly assigned to three experimental groups including sham-operated group, post-MI control group (AMI-Sed) and MI+exercises group(AMI-Ex). Rats were housed under sedentary conditions and assigned to 8-week treadmill exercise protocol.
Result: Comparing with AMI-Sed groups, in AMI-Ex group collagen volume fraction(CVF) of LV non-inforction area deceased and pulmonary congestion reduced(P<0.05), LVSP and +dp/dt max improved, but LVEDP increased(P<0.05). AMI-Ex group was associated with normalization of the MI-induced decrease in type Ⅰ procollagen and type Ⅲ procollagen mRNA levels (P<0.05). Also, it showed that exercise decreased MMP-2 protein expression in non-infarcted LV, but ET and MI had no effect on MMP-9 protein expressions in AMI-Ex rats.
Conclusion: Early ET in rats after AMI may have beneficial effect on LV remodeling and pumps function by attenuating type I and Ⅲ procollagen and MMP-2 expression. It suggests that early exercises training is a positive clinically relevant option in post-MI rehabilitation. |
| Keywords:myocardial infarction ventricular remodeling exercises training collagen matrix metalloproteinase-2 matrix metalloproteinase-9 |
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