| 彭峰林,张 林,陈德权,任 绮,邓树勋.间歇运动对缺血再灌注损伤大鼠心肌蛋白激酶C表达的影响[J].中国康复医学杂志,2010,25(6):544~547 |
| 间歇运动对缺血再灌注损伤大鼠心肌蛋白激酶C表达的影响 点此下载全文 |
| 彭峰林 张 林 陈德权 任 绮 邓树勋 |
| 广西师范大学体育学院，广西 桂林 541004 |
| 基金项目：广西教育厅科学研究项目、广西师范大学博士基金 |
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| 摘要
目的：探讨间歇运动对缺血再灌注（I/R）心肌中蛋白激酶C（PKC）表达的影响，为运动预处理机制研究提供依据。
方法：将32只大鼠随机分成4组：间歇运动训练组、一次间歇运动组、对照组和假手术组。I/R前，间歇运动训练组进行高强度间歇运动训练，一次间歇运动组仅进行一次高强度的间歇运动，对照组不运动。结扎大鼠左冠状动脉方法进行大鼠心肌缺血30min，松开结扎线再灌注40min。采用免疫组化方法和逆转录聚合酶链反应（RT-PCR）技术检测心肌细胞PKC的表达。
结果：间歇运动训练模型组、一次间歇运动模型组心肌PKC表达较对照模型组增强。
结论：间歇运动训练和一次间歇运动能激活PKC，增强PKC在I/R心肌的表达，间歇运动对I/R心脏的保护作用通过PKC途径介导，前者的影响更为显著。 |
| 关键词：间歇运动 缺血再灌注损伤 蛋白激酶C 信号转导 |
| The effect of interval exercises on expression of protein kinase C in rat′s ischemic-reperfusion cardiac myocyte Download Fulltext |
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| School of Physical Education, Guangxi Normal University, Guilin, 541004 |
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| Abstract: |
| Abstract
Objective：To explore the effect and mechanism of interval exercises in protein kinase C(PKC) mediated cardioprotection against ischemia-reperfusion(I/R) injury in rats.
Method：Thirty-two rats were used and randomly divided into four groups: chronic interval exercises training group (CIE, n=8), acute interval exercises group (AIE, n=8), control group (C, n=8) and sham operated group (S, n=8). The rats in CIE group conducted high intensity interval exercises training for 7 weeks, the rats in AIE group conducted high intensity interval exercises training only one time. The others had no any exercises training. Myocardial I/R injury was induced by the occlusion of left anterior descending branch of coronary artery in vivo. After 30min ischemia and 40min reperfusion, suitable myocardium were taken to examine PKC by immunohistochemical means, examine PKCmRNA expression by reverse transcription-polymerase chain reaction(RT-PCR) technique.
Result：Expressions of PKC in CIE group and AIE group were significant higher than C group.
Conclusion：Chronic interval exercises training and once acute interval exercises can efficiently activate PKC and increase its′ expression in I/R rats′ myocardium. Cardioprotection against I/R injury induced by chronic interval exercises training and once acute interval exercises may be mediated by PKC signal pathway. The effect of chronic interval exercises training showed more significant effect. |
| Keywords:interval exercises ischemia reperfusion injury protein kinase C signal transduction |
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