| 刘 浩.不同负荷游泳运动对小鼠心电活动和心肌血管内皮细胞生长因子表达的影响[J].中国康复医学杂志,2010,25(7):650~654 |
| 不同负荷游泳运动对小鼠心电活动和心肌血管内皮细胞生长因子表达的影响 点此下载全文 |
| 刘 浩 |
| 河南科技大学医学院解剖学与组织胚胎学教研室，河南洛阳， 471003 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 1956 |
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| 摘要： |
| 摘要
目的：探讨三种不同负荷对小鼠的心电活动、心肌组织中血管内皮细胞生长因子（VEGF）的表达以及体重的影响。
方法：雄性2月龄昆明小鼠30只，适应性喂养1周后，随机平均分为静养对照组、120min组、120min+负重组，对训练组进行游泳训练，观察各组的体重改变。训练完成后，连续监测、记录ECG各值，完成后取其心脏处理后观察VEGF的表达。利用IMAGE PRO PLUS 5.1图像分析软件测量面积比，心电活动主要测量心率、P-R间期、P波时程、P波幅度、P-R段、QRS波群时程、QRS波群峰峰值、T波时程、T波幅度及Q-T间期。
结果：三组小鼠中120min+负重组随着训练时间的延长而逐渐不能顺利地完成任务，游完后都筋疲力尽；而其他两组的小鼠活动状态一直较好。三组小鼠在8周的训练时间里，饮水和进食较好，体重一直呈现上升的趋势。静养对照组VEGF表达为阴性；120 min组表达为弱阳性；120min+负重组表达为阳性。P-R间期、P波时程、P波幅度、P-R段影响显著；QRS波群时程、QRS波群峰峰值、T波时程、T波幅度及Q-T间期影响不显著。
结论：运动强度和心脏的功能性代偿能力增强是成正比的，此结果为心脏康复中运动训练负荷的影响提供了试验依据。 |
| 关键词：运动负荷 小鼠 心电活动 心肌 血管内皮细胞生长因子 |
| Effects of swimming with different loads on electrocardioactivity and myocardial vascular endothelial cell growth factor in mouse Download Fulltext |
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| Dept. of Anatomy and Histology, Medical College, Henan University of Science and Technology, Luoyang Henan, 471003 |
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| Abstract: |
| Abstract
Objective：To investigate the effects of swimming with three different loads on vascular endothelial cell growth factor(VEGF) expressions in mice myocardium, electrocardioactivity and bodyweight.
Method：As experimental objects, after 1 week feeding adaptation 30 Kunming male mice were divided into 3 groups randomly: control group, 120min group and 120min+burdened group. After completing swimming training, mice hearts were removed and stained by HE and immunochemical technique. The changes of body weight and VEGF expression in mice myocardium were observed. IMAGE PRO PLUS 5.1 image analysis software was used to measure area ratio. In electrocardioactivity observation, heart rate, P-R interval, duration of P, amplitude of P, durations of P-R segment and QRS complex, QRS complex peak, duration of T, amplitude of T and Q-T interval were measured.
Result: With the prolongation of training time 120min+burdened group could not complete the tasks smoothly gradually, and displayed exhaustively after training. In the other two groups activities of mice were better. In eight weeks training three groups all drank and ate better, and their body weight showed an upward trend. The control group displayed negative VEGF expression, 120min group displayed weak positive expression and 120min+burdened group displayed positive recombinant expression. The effects on P-R interval, duration of P, amplitude of P and duration of P-R segment were significant；and the effects on QRS complex, QRS complex peak, duration of T, amplitude of T and Q-T interval were not significant.
Conclusion： The ability of functional compensation of heart is directly proportional to the intensity of exercises training. This study provide experimental evidence for the effects of exercises training load in cardiac rehabilitation. |
| Keywords:exercises load mouse electrocardioactivity myocardium vascular endothelial cell growth factor |
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