| 滕光寿,李 玲,袁 华,牟 翔,王冰水,刘 卫,陈景藻,曲丽莉,王兴亚.L-谷氨酰胺对次声致大鼠海马细胞凋亡和坏死的影响[J].中国康复医学杂志,2010,25(11):1044~1046 |
| L-谷氨酰胺对次声致大鼠海马细胞凋亡和坏死的影响 点此下载全文 |
| 滕光寿 李 玲 袁 华 牟 翔 王冰水 刘 卫 陈景藻 曲丽莉 王兴亚 |
| 第四军医大学唐都医院药剂科，西安，710038 |
| 基金项目：全军医学科学技术研究“十五”计划指令性课题(OlL071) |
| DOI： |
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| 摘要
目的：探讨L-谷氨酰胺（L-Gln）对次声暴露下大鼠海马细胞凋亡率和坏死率的影响。
方法：将60只雄性SD大鼠随机分为正常对照组、次声组、次声+药物组、药物→次声组，分别将次声组、次声+药物组、药物→次声组暴露于16Hz、130dB次声环境中，正常对照组也置于次声舱内，但期间不给予次声作用。经7d相应处理后测试各组大鼠海马细胞凋亡率和坏死率的变化。
结果：与正常对照组大鼠相比，次声组海马细胞凋亡率和坏死率显著升高（P＜0.01）；与次声组相比，次声+药物组、药物→次声组，海马细胞凋亡率和坏死率显著降低（P＜0.01）；与药物→次声组相比，次声+药物组海马细胞凋亡率和坏死率降低，但差异无显著性意义（P＞0.05）。
结论：次声（16Hz/130dB，2h/d，共7d）作用可引起大鼠海马细胞凋亡及坏死，导致海马细胞损伤；L-Gln能够部分有效预防和保护次声对大鼠海马细胞损伤的作用。 |
| 关键词：次声 L-谷氨酰胺 海马细胞 凋亡 坏死 |
| The effect of L-glutamine on rat hippocampal cells′ apoptosis and necrosis after infrasound exposure Download Fulltext |
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| Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi′an, 710038 |
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| Abstract: |
| Abstract
Objective: To study the effects of L-glutamine(L-gln) on rat hippocampal cells′ apoptosis and necrosis rates after infrasound exposure.
Method: Sixty male SD rats were randomly divided into 4 groups: normal control group, infrasound group (16Hz/130dB infrasound), infrasound + drug group (16Hz/130dB infrasound + L-Gln), drug→infrasound group (L-Gln for 7 days, and then exposed to 16Hz/130dB infrasound). No special treatment was performed on the normal control group, and the remained 3 groups were performed to L-Gln and/or infrasound treatment. 7d later, hippocampus was isolated and the cell suspension was collected to detect the apoptosis and necrosis rates of hippocampal cells.
Result: Compared with normal control group, apoptosis and necrosis rates of hippocampal cells in infrasound group increased significantly (P＜0.01). Compared with infrasound group, the rates of apoptosis and necrosis of hippocampal cells in infrasound + drug group and drug→infrasound group decreased significantly (P＜0.01). Compared with drug→infrasound group, it showed a lower rates of hippocampal cells apoptosis and necrosis in infrasound + drug group, but there was no significant difference between both groups(P＞0.05).
Concluson: Infrasound (16Hz/130dB, 2h/d, 7d) administration can induce apoptosis and necrosis of rat hippocampal cells and lead to cellullar damage in hippocampus; L-Gln can partly prevent and protect from the infrasound-induced injury on the rat hippocampus. |
| Keywords:infrasound L-glutamine hippocampal cell apoptosis necrosis |
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