| 张 杨,王永慧,丁欣利,王艳琴,王 荣,岳寿伟.TRPV4在介导大鼠背根神经节持续受压后机械和热痛敏中的作用[J].中国康复医学杂志,2010,25(12):1123~1130 |
| TRPV4在介导大鼠背根神经节持续受压后机械和热痛敏中的作用 点此下载全文 |
| 张 杨 王永慧 丁欣利 王艳琴 王 荣 岳寿伟 |
| 山东大学齐鲁医院康复科，济南，250012 |
| 基金项目：国家自然科学基金资助课题(30872732，81071597)；山东省自然科学基金资助课题(Z2007C04) |
| DOI： |
| 摘要点击次数: 2429 |
| 全文下载次数: 2505 |
| 摘要： |
| 摘要
目的：观察持续机械压迫(CCD)对瞬时感受器电位离子通道4（TRPV4）基因、蛋白表达及功能的影响，明确TRPV4是否参与CCD导致的机械和热痛敏。
方法：建立CCD模型后，分别于手术前及手术后第7天、第14天及第28天取材前测量运动功能、机械刺激缩爪反应阈值和热辐射刺激缩爪反应潜伏期。为了测量TRPV4反义核苷酸干扰对机械和热痛阈值的影响，在蛛网膜下腔内注入TRPV4寡脱氧核苷酸(ODN) 40μg/d，每天1次，第7天后测量大鼠行为学变化。使用实时定量RT-PCR检测TRPV4基因表达的变化，Western blot检测TRPV4蛋白质表达量的变化，激光共聚焦检测低渗溶液和佛波醇（4?琢-PDD）刺激背根神经节(DRG)神经元后细胞内钙离子浓度的变化。
结果：所有动物在损伤前后步态均正常，持续压迫明显降低大鼠的机械和热痛阈，TRPV4干扰可部分逆转该痛敏。持续机械压迫可以明显增加TRPV4基因和蛋白的表达，手术后第7天，第14天和第28天，TRPV4 mRNA的表达分别为假手术组大鼠的4.29倍、2.95倍和2.48倍, 蛋白表达量分别为假手术组大鼠的4.34倍，3.88倍和2.47倍。持续机械压迫后，对低渗溶液和4?琢-PDD产生反应的DRG神经元的比例数增加，细胞内钙的峰值增高。这种反应被TRPV4反义ODN所抑制。
结论：CCD可以上调TRPV4的基因、蛋白表达，敏化通道的功能；TRPV4参与介导CCD导致的机械和热痛敏。 |
| 关键词：机械性痛敏 热痛敏 瞬时感受器电位离子通道4 背根神经节 持续压迫 |
| The role of transient receptor potential vanilloid 4 in mediating mechanical and thermal allodynia following chronic compression of dorsal root ganglion in rats Download Fulltext |
|
| Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, Jinan, 250012 |
| Fund Project: |
| Abstract: |
| Abstract
Objective: To investigate the role of transient receptor potential vanilloid 4(TRPV4) in mediating mechanical and thermal allodynia in rodent models of chronic compression of dorsal root ganglion (CCD).
Method: The levels of TRPV4 mRNA and protein expressions in dorsal root ganglion (DRG) were assessed using real-time RT-PCR and Western blot analysis respectively at the 7th, 14th and 28th d post-CCD. The effects of spinal administration of TRPV4 antisense oligodeoxynucleotide(ODN) and mismatch ODN on CCD-induced mechanical and thermal allodynia were evaluated. The calcium responses to hypotonic solution and 4α-phorbol 12, 13-didecanoate (4α-PDD) were assessed following sham surgery, CCD, spinal application of TRPV4 antisense ODN and mismatch ODN.
Result: The levels of TRPV4 mRNA and protein expression increased significantly at the 7th-28th d post-CCD with the highest level at the 7th d post-CCD when compared with the sham group. TRPV4 antisense ODN, but not mismatch ODN, partly reversed the CCD-induced mechanical and thermal allodynia. Additionally, TRPV4 antisense ODN had no effect on the baseline nociceptive response. The percentage of DRG neurons responsive to hypotonic solution and 4α-PDD and the fluorescence ratio of calcium response also enhanced significantly in both CCD group and mismatch ODN group. These increased responses were significantly inhibited by TRPV4 antisense ODN.
Conclusion: TRPV4 plays a crucial role in CCD-induced mechanical and thermal allodynia. |
| Keywords:mechanical allodynia thermal allodynia transient receptor potential vanilloid 4 dorsal root ganglion chronic compression |
|
| 查看全文 查看/发表评论 |
