| 周治来,陈银海,靳安民,刘 巍.运动训练对脊髓损伤大鼠Nogo-A、NgR mRNA表达的影响[J].中国康复医学杂志,2011,26(2):143~147 |
| 运动训练对脊髓损伤大鼠Nogo-A、NgR mRNA表达的影响 点此下载全文 |
| 周治来 陈银海 靳安民 刘 巍 |
| 南方医科大学珠江医院骨科中心，广州，510282 |
| 基金项目：广东省自然科学基金项目（8451051501000460） |
| DOI： |
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| 摘要： |
| 摘要
目的：探讨运动训练对脊髓损伤（SCI）大鼠髓鞘源性神经抑制因子（Nogo-A）及其受体（NgR）mRNA表达的影响。
方法：44只SD大鼠随机分为运动训练组16只、损伤组16只及假手术组12只，采用改良Allens打击法制作大鼠T10脊髓损伤模型。造模后每周应用BBB评分法观察大鼠后肢运动功能, 在第8、10、14天处死大鼠，每组4只，以荧光定量PCR法测定不同时间点脊髓组织中Nogo-A与NgR mRNA相对含量。
结果：手术后第4—8周，运动训练组大鼠BBB评分明显高于SCI组；运动训练组与SCI组Nogo-A与NgR mRNA表达明显高于假手术组（P<0.05），随着时间推移，运动训练组与SCI组Nogo-A与NgR mRNA表达量趋于下降。术后第8、10天，SCI组Nogo-A mRNA表达均显著高于运动训练组与假手术组（P<0.05），至第14天，运动训练组与SCI组表达差异无显著性意义（P>0.05），但均高于假手术组（P<0.05），假手术组在各时间点表达差异均无显著性意义（P>0.05）；SCI组在各时间点NgR mRNA表达均高于运动训练组与假手术组（P<0.05），运动训练组在第10天便下降至与假手术组差异无显著性意义（P>0.05），假手术组在各时间点表达无差异（P>0.05）。
结论：康复训练能减少Nogo-A、NgR mRNA的表达，促进脊髓损伤大鼠后肢功能的恢复。 |
| 关键词：脊髓损伤 康复训练 Nogo-A NgR BBB评分 |
| Effects of exercise training on the expressions of Nogo-A、NgR mRNA in spinal cord injury rats Download Fulltext |
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| Dept． of Orthopedic，Zhujiang Hospital，Southern Medical University，Guangzhou，510282 |
| Fund Project: |
| Abstract: |
| Abstract
Objective:To explore the effects of exercise training on the expression of Nogo-A and its receptor NgR in spinal cord injury(SCI) rats.
Method: Forty-four Sprague-Dawley rats were assigned into two groups（test group 32 rats and sham-operated group 12 rats）randomly. Rats of test group experienced modified Allen impactor（H=25mm）at T10, then were randomly divided into 2 groups equally: training-treated group and SCI group. The hindlimbs movement was assessed by Basso-Beattie-Bresnahan(BBB) scale and the injured spinal cord tissue was extracted for Nogo-A and NgR mRNA examination through SYBR Green real-time PCR on the 8th，10th，14th day after SCI.
Result:Compared with SCI group，the BBB score in exercise training-treated group showed marked improvement between the 4th week and the 8th week after injury（P<0.05）,Compared with sham-operated group,the expressions of Nogo-A and NgR mRNA in training-treated group and SCI group increased significantly postoperation（P<0.05）,then declined gradually as time prolonged. The expression of Nogo-A mRNA in SCI group was significantly higher than that in training-treated group and sham-operated group on the 8th and 10th day postoperation（P<0.05）, there was no significant difference between SCI group and training-treated group on the 14th day, but all higher than that in sham-operated group at the three time points, there was no statistical difference among the three time points in sham-operated group. The expression NgR mRNA in SCI group was significant higher than that in the two other groups at three time points; otherwise, that in training-treated group decreased almost the same as that in sham-operated group on the 10th day postoperation（P>0.05）. There was also no statistical difference among the three time points in sham-operated group.
Conclusion: Exercise training could down-regulate the expressions of Nogo-A and NgR mRNA in SCI rats effcectively,and promote functional recovery of hindlimbs. |
| Keywords:spinal cord injury rehabilitation training Nogo-A NgR BBB score |
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