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安恒远,李雪萍,王大新,程 凯,林 强,林爱翠,高明霞.低强度脉冲超声波对软骨细胞中金属蛋白酶-13与Ⅱ型胶原的影响[J].中国康复医学杂志,2011,26(3):226~231
低强度脉冲超声波对软骨细胞中金属蛋白酶-13与Ⅱ型胶原的影响    点此下载全文
安恒远  李雪萍  王大新  程 凯  林 强  林爱翠  高明霞
南京医科大学附属南京第一医院康复医学科,江苏南京,210006
基金项目:国家973项目课题 (2007CB936104)
DOI:
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摘要:
      摘要 目的:研究低强度脉冲超声波(LIPUS)对体外培养软骨细胞中金属蛋白酶-13(MMP-13)与Ⅱ型胶原的影响。 方法:选用6只1月龄的新西兰大白兔膝关节软骨进行体外培养,传至第二代,分为对照组与4个LIPUS辐射组,LIPUS强度分别是20mW/cm2、30mW/cm2、40mW/cm2、50mW/cm2。每天辐射20min,连续10d,每天细胞计数;分别在第5天及第10天收集细胞,采用Western-blot技术、qRT-PCR技术检测软骨细胞中MMP-13、Ⅱ型胶原的含量,并分析两者之间的相关性。 结果:①与对照组相比,各 LIPUS辐射组软骨细胞数均明显增高(P<0.05),其中40mW/cm2组软骨细胞数最高,与其他各辐射组比较有显著性差异(P<0.05);②细胞培养5d和10d后,MMP-13含量均较第0天明显升高(P<0.05)。但各辐射组MMP-13含量均较其对照组显著降低(P<0.05);其中40mW/cm2组MMP-13含量最低(P<0.05);③细胞培养5d和10d后,Ⅱ型胶原表达量均较第0天明显降低(P<0.05)。但各辐射组Ⅱ型胶原表达量均较其对照组显著升高(P<0.05)。其中40mW/cm2组Ⅱ型胶原表达量最高(P<0.05);④MMP-13与Ⅱ型胶原含量呈负相关(r=-0.757,P<0.05)。 结论:不同强度的LIPUS体外辐射可促进软骨细胞增殖,抑制MMP-13的表达,延缓II型胶原的降解。
关键词:低强度脉冲超声波  软骨细胞  金属蛋白酶  Ⅱ型胶原
Effects of low intensity pulsed ultrasound on MMP-13 and type II collagen of chondrocytes    Download Fulltext
Nanjing First Hospital Affiliated to Nanjing Medical University,Nanjing,210006
Fund Project:
Abstract:
      Abstract Objective:To study the effects of low intensity pulsed ultrasound(LIPUS) on matrix metalloproteinase(MMP-13) and type II collagen of chondrocytes. Method: The chondrocytes were obtained from knee joints of six one-month old rabbits. Chondrocytes were divided into 5 groups:control group and 4 LIPUS groups with intensity of 20mW/cm2,30mW/cm2,40mW/cm2,50mW/cm2 respectively.During the next cultured 10d, the chondrocytes were irradiated 20min and cells were counted every day.On the 5th d and 10th d, cells were collected and the MMP-13 and type II collagen content were detected by Western-blot and qRT-PCR. The correlation between MMP-13 and type II collagen was analyzed. Result: ①Contrasting with control group, the chondrocytes number was higher in each LIPUS group(P<0.05) and the number was significantly higher in 40mW/cm2 group (P<0.05).②MMP-13 contents were higher at the 5th d and 10th d than that at the 0d(P<0.05).After irradiated 5d and 10d, the content of MMP-13 in each LIPUS group was remarkable lower than that in control group(P<0.05) and in 40mW/cm2 group the content of MMP-13 was the lowest (P<0.05).③After cultured 5d and 10d, type Ⅱ collagen content was lower than that at the 0d(P<0.05). The content of type Ⅱ collagen in each LIPUS groups was remarkable higher than that in control group(P<0.05) and in 40mW/cm2 group the content of typeⅡ collagen was the highest (P<0.05).④The correlation between MMP-13 and type Ⅱ collagen content was negative correlation (r=-0.757,P<0.05). Conclusion: Different intensity of LIPUS could promote chondrocyte proliferation and inhibit the expression of MMP-13 and postpone the degradation of typeⅡ collagen.
Keywords:low intensity pulsed ultrasound  chondrocytes  matrix metalloproteinase  type II collagen
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