| 赵 红,张倩茹,卢晓梅,张海鹏,陈学新.高压氧对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1、基质金属蛋白酶-9表达的影响[J].中国康复医学杂志,2011,26(6):550~554 |
| 高压氧对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1、基质金属蛋白酶-9表达的影响 点此下载全文 |
| 赵 红 张倩茹 卢晓梅 张海鹏 陈学新 |
| 中国医科大学基础医学院机能实验中心，沈阳市和平区北二马路92号，110001 |
| 基金项目：辽宁省教育厅高等学校科研基金资助项目 （2005L456）；辽宁省科技厅博士启动基金资助项目（20101149） |
| DOI： |
| 摘要点击次数: 2232 |
| 全文下载次数: 2819 |
| 摘要： |
| 摘要
目的：研究高压氧（HBO）对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1（Cav-1）、MMP-9表达的影响。
方法：雄性Wistar大鼠，随机分成假手术组、脑缺血再灌注组(IR)、IR+HBO、HBO组。复制局灶性脑缺血再灌注模型。HBO和IR+HBO组经0.25MPaHBO治疗5次。采用比色法、免疫组化法及Western 印迹法检测血脑屏障的通透性和Cav-1、基质金属蛋白酶-9（MMP-9）蛋白的表达。
结果：IR组第4、12、24、48、72小时脑组织伊文思蓝(EB)的含量与第0小时组相比明显增加，再灌注后第4小时EB的含量最高。IR+HBO组脑组织EB的含量明显低于IR组。IR组第4、12、24、48、72小时 Cav-1、MMP-9蛋白表达明显高于0小时组。IR+HBO组与IR组相比脑组织Cav-1、MMP-9蛋白表达显著减低。
结论：HBO降低了脑缺血再灌注时增高的脑微血管内皮细胞Cav-1蛋白和MMP-9的表达。这可能是HBO对脑缺血再灌注时血脑屏障通透性起保护作用的机制之一。 |
| 关键词：高压氧 脑缺血再灌注 小窝蛋白-1 基质金属蛋白酶-9 |
| Effects of hyperbaric oxygen on caveolin-1 and matrix metalloproteinase-9 in brain tissues after cerebral focal ischemia-reperfusion in rats Download Fulltext |
|
| College of Basic Medical Sciences, China Medical University, Shenyang，110001 |
| Fund Project: |
| Abstract: |
| Abstract
Objective: To investigate the effect of hyperbaric oxygen (HBO) on caveolin-1(Cav-1) and matrix metalloproteinase-9(MMP-9) in brain tissues after cerebral focal ischemia-reperfusion in rats.
Method: Male Wistar rat were randomly assigned into sham, ischemia-reperfusion (IR), HBO, and IR+HBO groups. On cerebral focal ischemia-reperfusion models, 0.25MPa HBO were applied 5 times, and 2% Evens blue (EB) was injected into tail veins 1h before execution. Expressions of Cav-1 and MMP-9, contents of EB in brain tissues were determined by Western blot technique, immunohistochemistry, and spectrophotometer, respectively.
Result: In IR group, EB content in brain tissues increased significantly compared with 0h, reached it's peak at the 4th h(P<0.01). EB content in IR+HBO group was significantly lower than that in IR group. While expressions of Cav-1 and MMP-9 augmented significantly by IR injury at the 4thh, 12thh, 24thh, 48thh, and 72ndh. Compared to IR group, HBO intervention reduced their corresponding expressions levels.
Conclusion: HBO intervention can reverse the increase of Cav-1 and MMP-9 caused by IR injury, which maybe one of the mechanisms for the protective effect of HBO on the permeability of blood-brain barrier in IR injury |
| Keywords:hyperbaric oxygen cerebral ischemia-reperfusion caveolin-1 matrix metalloproteinase-9 |
|
| 查看全文 查看/发表评论 |
|
|
|
|
|
