| 薄 海,张红英,李海英,张 勇.低氧复合运动对大鼠骨骼肌解偶联蛋白3表达及线粒体功能的影响[J].中国康复医学杂志,2012,27(1):16~21 |
| 低氧复合运动对大鼠骨骼肌解偶联蛋白3表达及线粒体功能的影响 点此下载全文 |
| 薄 海 张红英 李海英 张 勇 |
| 天津武警后勤学院部队训练医学教研室，天津，300162 |
| 基金项目：国家自然科学基金项目（30270638）；武警医学院科研基金（WY2009-11) |
| DOI： |
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| 摘要
目的：观察单纯低氧及低氧复合运动对大鼠骨骼肌线粒体解偶联蛋白3（UCP3）表达的影响，并探讨其对线粒体能量转换和活性氧（ROS）生成的影响。
方法：30只健康雄性SD大鼠随机分为：常氧对照组（NC, n=10）、单纯低氧组（HC, n=10）和低氧复合运动训练组（HT, n=10）。低氧干预为常压低氧帐篷，模拟11.3%的氧浓度，运动干预为低氧帐篷内53% VO2max强度的跑台训练，1h/d。4周后测定线粒体呼吸功能、ATP合成酶活力、ROS生成速率、UCP3 mRNA和UCP3蛋白表达。
结果：HC与NC组比较，态3呼吸速率（ST3）、呼吸控制比（RCR）、磷氧比（ADP/O）、ATP合成酶活力、UCP3 mRNA和蛋白表达均显著降低（P＜0.05—0.01），ROS生成速率升高（P＜0.05）。HT与HC组比较，ST3、RCR、ADP/O、ATP合成酶活力、UCP3 mRNA和蛋白表达均显著升高（P＜0.05—0.01），ROS生成速率降低（P＜0.05）。
结论：低氧复合运动可上调骨骼肌线粒体UCP3表达，抑制ROS过度生成，并通过上调ATP合成酶活力，保持线粒体能量转换效率。 |
| 关键词：低氧 解偶联蛋白3 线粒体能量转换 活性氧 骨骼肌 |
| Effect of hypoxia combined exercise training on uncoupling protein 3 expression and mitochondrial function in skeletal muscles of rats Download Fulltext |
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| Logistics College of Chinese People's Armed Police Force, Tianjin, 300162 |
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| Abstract: |
| Abstract
Objective: To observe the effect of hypoxia alone and hypoxia combined with exercise training on mitochondrial energy transferring and reactive oxygen species (ROS) generation, and to identify roles of uncoupling protein 3 (UCP3) in skeletal muscles of rats.
Method: A total 30 healthy male Sprague-Dawley rats were randomly divided into three groups: normoxia control group (NC, n=10), hypoxia control group (HC, n=10), and hypoxia combined exercise training group (HT, n=10). The hypoxia animals were subjected to hypoxia exposure in normobaric hypoxic tent simulating 11.3% oxygen concentration. The exercise training animals exercised on a motor-driven rodent treadmill in normobaric hypoxic tent for 1h/d, at an intensity of 53% VO2max. After 4 weeks, mitochondrial respiration parameters, ATP synthetase activity, ROS generation rate, UCP3 mRNA and UCP3 protein expression were measured.
Result: Comparing with NC group, in HC group, state 3 respiratory rate(ST3), respiratory control ratio(RCR), ratio of ADD to oxygen(ADP/O), ATP synthetase activity, UCP3 mRNA and protein expression showed dramatic reduce(P＜0.05—0.01). And H2O2 generation elevated significantly(P＜0.05). Comparing with HC group, in HT group, ST3, RCR, ADP/O, ATP synthetase activity, UCP3 mRNA and protein expression improved significantly(P＜0.05—0.01). And H2O2 generation decreased (P＜0.05).
Conclusion: Hypoxia combined exercise training could markedly enhance mitochondrial UCP3 expression and ATP synthetase activity, which suppressed mitochondrial ROS generation and maintained mitochondrial energy transferring efficiency. |
| Keywords:hypoxia uncoupling protein 3 mitochondrial energy transferring reactive oxygen skeletal muscle |
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