| 钟艺华,李光勤,唐显军,黄浩然.电针对局灶性脑梗死大鼠神经功能及LINGO1表达的穴位比较研究[J].中国康复医学杂志,2012,27(5):410~414 |
| 电针对局灶性脑梗死大鼠神经功能及LINGO1表达的穴位比较研究 点此下载全文 |
| 钟艺华 李光勤 唐显军 黄浩然 |
| 重庆医科大学附属第一医院神经内科，重庆，400016 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 1948 |
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| 摘要： |
| 摘要
目的:比较督脉取穴法（人中-百会穴）与督脉取穴法联合背俞取穴法（人中-百会穴+肝俞-肾俞穴）在局灶性脑缺血大鼠的治疗效果上是否存在差异。
方法:将健康成年雄性SD大鼠91只随机分为4组：假手术组、模型组、治疗A组（电针人中-百会穴）、治疗B组（电针人中-百会穴+肝俞-肾俞穴），采用线栓法制作大鼠右侧大脑中动脉闭塞模型（MCAO）。电针刺激在造模成功90min后开始，30min/d。分别选取缺血第1、3、7及14天共4个时间点，运用Longa评分对神经功能恢复情况进行评价，RT-PCR及免疫组化测定缺血侧皮质LINGO1 mRNA和蛋白表达。
结果：假手术组无神经功能障碍。治疗A组和治疗B组运动功能恢复情况较模型组明显改善（P<0.05）；LINGO1 mRNA和蛋白表达在模型组中缺血第1天时明显增强第3—14天表达逐渐下降，但第14天时仍较假手术组高（P<0.01）；治疗A组和治疗B组在第7—14天时其LINGO1 mRNA和蛋白表达均较模型组低（P<0.05），并且，治疗B组在7—14天时的LINGO1蛋白表达较治疗A组低，二者之间差异有显著性意义（P<0.05）。
结论：局灶性脑梗死大鼠缺血侧脑皮质LINGO1 mRNA和蛋白表达明显上调，在脑梗死急性期给予电针刺激后能下调其表达水平，促进轴突再生，加速神经功能恢复；联合取穴法与单一取穴法比较，能取得更好的治疗效果。 |
| 关键词：电针 脑梗死 LINGO1蛋白 轴突再生 |
| A comparative study of acupoints by electroacupuncture on neural function and expression of LINGO1 after focal cerebral infarction in rats Download Fulltext |
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| Department of Neurology，the First Affiliated Hospital，Chongqing Medical University Chongqing, 400016 |
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| Abstract: |
| Abstract
Objective：To investigate the difference between the effect of electroacupuncture（EA）at “Renzhong-Baihui”（DU26-DU20,RB）and“Renzhong-Baihui+Ganshu-Shenshu”（DU26-DU20+BL18-BL23，RB+GS）after focal cerebral infarction in rats.
Method：The healthy adult male SD rats（n=91） were randomly divided into sham-operated group，model group，treatment group A（RB）and treatment group B（RB+GS） and subjected to establish the model of middle cerebral artery occlusion（MCAO）on the right side by nylon monofilament suture. EA was performed at 90 min after establishing of successful model，30min/d. The neural function recovery was evaluated by Longa's score，RT-PCR and immunohistochemistry techniques were used to detect the expressions of LINGO1 mRNA and protein in ischemic cortex on the 1st、3rd、7th and 14th d respectively after ischemia.
Result： The sham-operated group had no neurological dysfunction. Motor function recovered significantly in treatment group A and treatment group B， compared with model group（P<0.05）. The expressions of LINGO1 mRNA and protein in model group enhanced obviously on the 1st d，then gradually decreased on the 3rd—14th d，but were still higher than that in sham-operated group on the 14th d（P<0.01）. The expressions of LINGO1 mRNA and protein in treatment group A and treatment group B were all lower than that in model group on the 7d—14d（P<0.05）；in addition，the expression of LINGO1 protein in treatment group B was lower than that in treatment group A，there was significant statistical difference between two treatment groups（P<0.05）.
Conclusion：The expressions of LINGO1 mRNA and protein in ischemic cortex increased significantly. EA might induce its' down-expression after acute cerebral infarction，promote axonal regeneration and accelerate the neural functional recovery. The therapeutic effect of RB+GS group was better than that of RB group. |
| Keywords:electroacupuncture cerebral infarction LINGO1 protein axon regeneration |
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