| 谢康玲,刘遂心,蔡 颖,张文亮.长期中等强度运动对小鼠骨骼肌HIF-1α mRNA的表达及葡萄糖转运的影响[J].中国康复医学杂志,2012,27(6):514~518 |
| 长期中等强度运动对小鼠骨骼肌HIF-1α mRNA的表达及葡萄糖转运的影响 点此下载全文 |
| 谢康玲 刘遂心 蔡 颖 张文亮 |
| 中南大学湘雅医院康复医学科，长沙，410008 |
| 基金项目：国家自然科学基金资助项目（30672213） |
| DOI： |
| 摘要点击次数: 2541 |
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| 摘要： |
| 摘要
目的：观察长期中等强度运动对小鼠骨骼肌血管内皮生长因子-A（VEGF-A）、葡萄糖转运体1(GLUT-1)、葡萄糖转运体4（GLUT-4）mRNA表达的影响，在此基础上进一步观察运动对缺氧诱导因子1α（HIF-1α）mRNA表达的影响，初步探讨运动对小鼠骨骼肌葡萄糖转运的影响及其可能机制。
方法：健康雄性昆明小鼠24只随机分为运动组和对照组，运动组小鼠给予12周中等强度跑步训练。末次运动后处死所有小鼠，取双侧腓肠肌，光镜及电镜观察显微及超微结构；实时 PCR法测定小鼠骨骼肌VEGF-A、GLUT-1、GLUT-4及HIF-1α mRNA的表达。
结果：①运动组小鼠较对照组体重明显增加（P＜0.05）；②骨骼肌肌细胞数目增加、肌纤维增粗，肌原纤维的直径增加，线粒体数目增加；③运动组小鼠骨骼肌VEGF-A、GLUT-1、GLUT-4及HIF-1α mRNA表达明显高于对照组（分别增加1.68、1.14、2.31、1.92倍，P分别＜0.05、0.01、0.01、0.01）。
结论：长期中等强度运动可导致小鼠体重增加、肌纤维增粗、线粒体数目增加，VEGF-A mRNA表达增加；可增加骨骼肌GLUT-1、GLUT-4 mRNA的表达，其机制可能与上调骨骼肌HIF-1α mRNA表达有关。 |
| 关键词：运动 缺氧诱导因子1α 骨骼肌 葡萄糖转运体 小鼠 |
| Effects of long-term exercise with moderate intensity on the expressions of hypoxia inducible factor-1α mRNA and glucose transporters in skeletal muscles of mice Download Fulltext |
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| Xiangya Hospital of Central South University, Changsha, 410008 |
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| Abstract: |
| Abstract
Objective: To investigate the effect of long-term exercise with moderate intensity on the expressions of glucose transporter-1 (GLUT-1) and glucose transporter-4 (GLUT-4) mRNA, as well as of hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor-A (VEGF-A) mRNA in skeletal muscles of mice and explore its possible mechanisms.
Method: Kunming male mice (n=24) were randomly divided into exercise group and control group. The mice in exercise group were forced to run with moderate intensity on a treadmill. After 12 weeks of exercise, all mice were sacrificed immediately and both sides of gastrocnemius were harvested. The middle part of tissue samples were prepared for H & E staining， and analysed under light microscope and transmission electron microscope. In addition, part of tissues were used to check gene expression by real-time PCR.
Result: ①Compared to control group, the body weights of exercise group increased significantly(P＜0.05). ②The number of muscle cells increased, the size of muscle fibers thickened, the diameter of myofibril and the amount of mitochondria increased significantly. ③The expressions of VEGF-A mRNA, GLUT-1 mRNA, GLUT-4 mRNA, and HIF-1α mRNA in exercise group were significantly higher (increased by 1.68, 1.14, 2.31 and 1.92-fold separately, P＜0.05, 0.01, 0.01, 0.01 separately) than those of control group.
Conclusion: ①Long-term exercise with moderate intensity can cause weight gain, thickening of skeletal muscle fibers, increasing of mitochondria amount and expression of VEGF-A mRNA; ②Long-term exercise with moderate intensity can increase the expressions of GLUT-1 mRNA, GLUT-4 mRNA in skeletal muscles. Induction of HIF-1α mRNA expression in skeletal muscles by exercise might be one of the possible mechanisms. |
| Keywords:exercise hypoxia inducible factor-1α skeletal muscle glucose transporter mouse |
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