| 房春燕,王 燕,王亚南,孟 蒂,张广学,李 宁.伏核内勿动蛋白A和降钙素基因相关肽1受体在炎性痛大鼠痛觉调制过程中的角色[J].中国康复医学杂志,2013,28(1):9~12 |
| 伏核内勿动蛋白A和降钙素基因相关肽1受体在炎性痛大鼠痛觉调制过程中的角色 点此下载全文 |
| 房春燕 王 燕 王亚南 孟 蒂 张广学 李 宁 |
| 潍坊医学院应用药理学实验室，261053 |
| 基金项目：山东省自然科学基金资助项目（ZR2009CL049） |
| DOI： |
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| 摘要
目的：探讨大鼠伏核内勿动蛋白A（Nogo-A）和降钙素基因相关肽1（CGRP1）受体在痛觉调制过程中的作用。
方法：健康雄性SD大鼠随机分为4组：空白对照组，伏核内注射生理盐水1μl；炎性痛模型对照组、吗啡组和纳洛酮组。福尔马林致炎成功后，分别向其伏核内注射生理盐水、吗啡、纳洛酮各1μl。采用Western blot法，观察各组大鼠伏核内Nogo-A和CGRP1受体蛋白表达的变化，分析炎性痛、吗啡、纳洛酮对大鼠伏核内Nogo-A和CGRP1受体蛋白表达的影响。
结果：正常大鼠伏核内有Nogo-A和CGRP1受体蛋白表达；福尔马林致炎后Nogo-A的蛋白表达量降低（P<0.05），而CGRP1表达升高（P<0.05）；与炎性痛模型对照组比较，吗啡组大鼠伏核内Nogo-A的蛋白表达量增高（P<0.05），CGRP1降低（P<0.05），而纳洛酮组Nogo-A降低（P<0.05），CGRP1增高（P<0.05）。
结论：Nogo-A和CGRP参与了炎症致痛敏大鼠伏核内的痛觉调制过程，且二者之间存在反向调节关系，内源性阿片系统可能参与并影响了该过程。 |
| 关键词：勿动蛋白-A 降钙素基因相关肽受体 吗啡 伏核 痛觉调制 |
| Role of neurite outgrowth inhibitor-A and calcitonin gene related peptide 1 receptor in pain modulation in nucleus accumbens of rats with inflammatory pain Download Fulltext |
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| Pharmacology Laboratory of Weifang Medical College, 261053 |
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| Abstract: |
| Abstract
Objective: To investigate the role of neurite outgrowth inhibitor-A(Nogo-A) and calcitonin gene related peptide 1(CGRP1) receptor in pain modulation in nucleus accumbens of rats.
Method: Healthy male SD rats were randomly divided into four groups： blank control group (saline was injected to the nucleus accumbens for 1μl); inflammatory pain model control group (formalin was injected to rat's hindpaw to induce inflammatory pain); morphine group and naloxone group (after inflammatory pain models were prepared, morphine and naloxone were injected to nucleus accumbens for 1μl respectively). The Western blot technique was used for Nogo-A and CGRP1 receptor protein recording. The influence of inflammatory pain, morphine and naloxone on Nogo-A and CGRP1 receptor protein expression in nucleus accumbens were analyzed.
Result: Nogo-A and CGRP1 receptor proteins expressed in nucleus accumbens of normal rats; the expression of Nogo-A protein decreased significantly（P<0.05）and CGRP1 receptor protein increased significantly（P<0.05）after formalin-induced inflammation. Compared with inflammatory pain model control group, the expression of Nogo-A protein increased significantly（P<0.05）and CGRP1 receptor protein decreased significantly（P<0.05）in morphine group; the expression of Nogo-A protein decreased significantly（P<0.05）and CGRP1 receptor protein increased significantly（P<0.05） in naloxone group.
Conclusion: Nogo-A and CGRP were both involved in pain modulation in nucleus accumbens of inflammatory pain rats, and the relationship between Nogo-A and CGRP was negative regulation. Endogenous opioid systems may be influence the role of Nogo-A and CGRP in pain modulation. |
| Keywords:neurite outgrowth inhibitor-A calcitonin gene related peptide receptor morphine nucleus accumbens pain modulation |
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