| 李卫平,胥方元,蹇 睿,王江林.电针对实验性兔膝骨关节炎模型白细胞介素-1β和基质金属蛋白酶-1表达的影响[J].中国康复医学杂志,2013,28(2):139~142 |
| 电针对实验性兔膝骨关节炎模型白细胞介素-1β和基质金属蛋白酶-1表达的影响 点此下载全文 |
| 李卫平 胥方元 蹇 睿 王江林 |
| 泸州医学院附属医院康复科，泸州，646000 |
| 基金项目：四川省卫生厅科研基金资助项目（050075） |
| DOI： |
| 摘要点击次数: 1781 |
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| 摘要： |
| 摘要
目的：观察电针治疗前后兔膝骨关节炎（KOA）模型关节冲洗液中细胞因子白介素-1β（IL-1β）和软骨中基质金属蛋白酶-1（MMP-1）表达的变化，探讨电针治疗KOA的作用机制。
方法：将30只新西兰大耳兔随机分为正常组、模型组及电针组。正常组为对照组，模型组和电针治疗组采用左膝关节伸直位管型石膏固定6周制作KOA模型，电针治疗组电针治疗14d。分别观察各组兔造模结束和治疗后左膝关节冲洗液中IL-1β含量变化，治疗后各组兔左股骨内侧髁软骨MMP-1表达的变化。
结果：采用放免法行关节冲洗液IL-1β含量测定，造模结束后正常组与模型组、电针治疗组组间比较，其后两者明显高于前者(P＜0.05)，治疗结束后模型组、电针治疗组组间比较，前者显著高于后者(P＜0.05)。治疗结束后软骨免疫组化法MMP-1测定，模型组和电针治疗组的MMP-1阳性率明显高于正常组（P＜0.05），且模型组MMP-1阳性率高于电针治疗组(P＜0.05)。
结论：电针能有效治疗KOA，其作用机制可能是电针使KOA关节中细胞因子IL-1β和软骨中MMP-1的含量减少，从而降低Ⅱ型胶原和蛋白聚糖的降解，促进新的Ⅱ型胶原和蛋白聚糖合成，改善血液循环，促进经气运行，从而有利于关节渗出液吸收，促进软骨的修复。 |
| 关键词：电针 骨关节炎 白细胞介素-1β 基质金属蛋白酶-1 |
| Effects of electro-acupuncture on expressions of interleukin-1β and matrix metalloproteinase-1 in rabbit knee osteoarthritis model Download Fulltext |
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| The Affiliated Hospital of Luzhou Medical College, Luzhou, 646000 |
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| Abstract: |
| Abstract
Objective: To observe the changes of interleukin-1β(IL-1β) from joint irrigation and matrix metalloproteinase-1(MMP-1) from cartilage expressed in rabbit's knee osteoarthritis(KOA) model before and after electro-acupuncture(EA) treatment, to explore the mechanism of EA treatment on knee osteoarthritis.
Method: Thirty healthy New Zealand big-ear rabbits were randomly divided into normal group, model group and EA group. In the latter two groups left knees were fixed in extension position with plaster cast for 6 weeks to establish KOA models. The EA group was treated for 14d. The changes of content of IL-1β expression in joint irrigation after modeling and treatment and changes of MMP-1 expression in cartilage of left condylus after treatment were observed respectively.
Result: The radioimmunoassay detection results revealed IL-1β expression in joint irrigation of model group and EA group after modeling were significantly higher than that of control group (P＜0.05), while the IL-1β expression in model group was significantly higher than that in EA group after treatment (P＜0.05). By immunohistochemical detection, the MMP-1 positive rates of model group and EA group were significantly higher than that of control group (P＜0.05), with a higher rate in model group(P<0.05).
Conclusion: EA treatment can effectively improve the rabbit's KOA, the mechanism of which is probably related with IL-1β reduction in joint and MMP-1 decrease in cartilage so as to, prevent the collagen Ⅱ and proteoglycan from degradation, promote new collagen Ⅱ and proteoglycan formation, improve blood and qi circulation, thus promote the absorption of joint exudates and cartilage repair. |
| Keywords:electro-acupuncture osteoarthritis interleukin-1β matrix metalloproteinase-1 |
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