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陈国仙,陈建庭,黄文华,郑 帅,王国荣,林宗锦,李国山.不同频率振动应力对破骨细胞特异性基因及骨保护素/核因子κB受体活化因子配体表达的影响[J].中国康复医学杂志,2014,29(2):99~103
不同频率振动应力对破骨细胞特异性基因及骨保护素/核因子κB受体活化因子配体表达的影响    点此下载全文
陈国仙  陈建庭  黄文华  郑 帅  王国荣  林宗锦  李国山
南方医科大学南方医院脊柱骨科,广州,510515
基金项目:福建省卫生厅青年基金资助项目(2011-2-49);福建莆田市科技局资助项目(2011D02)
DOI:
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摘要:
      摘要 目的:观察不同频率振动应力对RAW264.7细胞体外诱导分化过程中,其特异性基因及骨保护素(OPG)/核因子κB受体活化因子配体(RANKL)表达的影响。 方法:应用复合振动仪,将不同频段3—10Hz、15—35Hz、35—45Hz、50—70Hz和70—90Hz振动应变分别作用于体外诱导分化的RAW264.7细胞,分别为B、C、D、E、F组,未进行振动干预组为A组,振动应变加载3天和6天时,应用RT-PCR方法检测破骨细胞特异性基因(TRAP、MMP-9和CATK)与OPG/RANKL的表达水平。 结果:不同振动频率组破骨细胞特异性基因表达水平逐渐减低,同时B、C、D组逐渐上调OPG基因表达,而RANKL基因的表达逐渐下调。 结论:不同频率振动应力均抑制RAW264.7细胞向成熟破骨细胞增殖分化。
关键词:振动应力  RAW264.7细胞  分化  破骨细胞  骨保护素  骨质疏松
Effects of different frequency of vibration strain on the expressions of osteoclast-specific genes and osteoprotegerin/receptor of activator for nuclear factor-κB ligand in osteoclast    Download Fulltext
Department of Orthopedics,Nanfang Hospital,Southern Medical University,Guangzhou,510515
Fund Project:
Abstract:
      Abstract Objective: To investigate effects of different frequency of vibration strain on the expression of osteoclast-specific genes and osteoprotegerin(OPG)/receptor of activator for nuclear factor-κB ligand(RANKL) in RAW264.7 cells in vitro induced differentiation process. Method: RAW264.7 cells were subjected to vibration strain with different frequency [3—10Hz (B-goup), 15-35Hz(C-group), 35—45Hz(D-group), 50—70Hz(E-group),70—90Hz(F-group)] and similar induced fluid. A-group cells were not subjected to vibration strain.The expressions of osteoclast-specific genes(TRAP, MMP-9 and CATK) and OPG/RANKL in osteoclasts were analyzed by semi-quantative RT-PCR. Result: In different frequency vibration groups, the expression levels of osteoclast-specific genes reduced gradually, at the same time in B、C、D group vibration strain promote the expression of OPG mRNA and inhibit the expression of RANKL mRNA gradually. Conclusion: Different frequency of vibration strain can inhibit proliferation and differentiation of RAW264.7 cells into osteoclasts.
Keywords:vibration strain  RAW264.7 cell  differentiation  osteoclast  osteoprotegerin  osteoporosis
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