| 何兰英,张 蓓,罗 勇,董为伟,王咏龙.电刺激小脑顶核对局灶脑缺血/再灌注后大鼠脑组织NF-кB、PPARγ、IкBα和COX-2 mRNA表达的影响[J].中国康复医学杂志,2014,29(2):107~112 |
| 电刺激小脑顶核对局灶脑缺血/再灌注后大鼠脑组织NF-кB、PPARγ、IкBα和COX-2 mRNA表达的影响 点此下载全文 |
| 何兰英 张 蓓 罗 勇 董为伟 王咏龙 |
| 成都市第二人民医院神经内科，成都，610017 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 1520 |
| 全文下载次数: 1889 |
| 摘要： |
| 摘要
目的：观察电刺激小脑顶核（FNS）对大鼠局灶脑缺血/再灌注（I/R）大脑缺血侧皮质PPARγ、IкBα和NF-кB P65蛋白表达的变化，以及对下游炎症因子COX-2 mRNA表达的影响，探讨FNS对大鼠局灶脑缺血/再灌注后脑保护作用的机制。
方法：采用SD大鼠建立局灶I/R模型，随机分为6组。正常对照组（NC组）、缺血再灌注组（I/R组）、缺血再灌注后小脑顶核刺激组（FNS组），根据再灌注时间不同分为7d和14d两个亚组。采用免疫组织化学法检测NF-κB P65蛋白表达，分别采用Western blotting和逆转录—聚合酶链反应法（RT-PCR）检测PPARγ、IкBα蛋白和COX-2 mRNA表达，同时检测各组脑梗死体积。
结果：免疫组织化学显示I/R 7d组和I/R 14d组NF-κB P65、PPARγ和IкBα蛋白较正常组显著增加（P＜0.05），FNS组NF-κB P65蛋白表达显著低于相应I/R组（P＜0.05），Western blotting检测显示FNS组PPARγ、IкBα蛋白表达明显高于相应正常组及I/R组（P＜0.05），RT-PCR显示FNS组COX-2 mRNA表达较I/R组显著降低（P＜0.05），而FNS组脑梗死体积较单纯I/R组明显减小（P＜0.05）。
结论：FNS可有效提高脑缺血/再灌注后PAARγ及IкBα表达，抑制由NF-κB P65调控的下游炎症因子COX-2 mRNA的表达，减轻脑梗死体积，这可能是FNS发挥中枢神经保护的机制之一。 |
| 关键词：电刺激 小脑顶核 NF-κB 脑缺血/再灌注 PAARγ COX-2 IкBα |
| Effects of electrical stimulation to cerebellar fastigial nucleus on expressions of NF-кB, PPARγ, IкBα and COX-2 mRNA under cerebral ischemia/reperfusion in rats Download Fulltext |
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| Department of Neurology, the Second People's Hospital of Chengdu, Chengdu, 610017 |
| Fund Project: |
| Abstract: |
| Abstract
Objective: To investigate the effect of electrical stimulation to cerebellar fastigial nucleus（FNS） on expressions of NF-кB P65, PPARγ, IкBα and COX-2 mRNA in rats brain after cerebral ischemia/reperfusion（I/R）, and explore the neuroprotection mechanism.
Method: A focal cerebral I/R model was established by middle cerebral artery occlusion (MCAO), and the rats were randomly divided into normal control group (NC group), cerebral ischemia reperfusion group (I/R group), I/R+FNS group（FNS group）. Infarct volume was measured, the protein of NF-кB P65 in rats brain was detected by immunohistochemistry, and the protein of PPARγ, IкBα was detected by Western blotting. The expression of COX-2 mRNA was detected by RT-PCR.
Result: Compared with NC group, the expressions of P65, PPARγ, IкBα protein and COX-2 mRNA increased under the I/R (P＜0.05), FNS could effectively inhibit the expression levels of P65 protein and COX-2 mRNA (P＜0.05). FNS could effectively induce the expressions of PPARγ, IкBα protein (P＜0.05), and reduce infarct volume significantly(P＜0.05).
Conclusion: FNS could effectively induce the expressions of PPARγ, IкBα protein, and effectively inhibit the expressions of P65 protein and COX-2 mRNA, and reduce infarct volume, which may be one of the mechanisms of its neuroprotective function on central nervous system. |
| Keywords:electrical stimulation cerebellar fastigial nucleus focal cerebral ischemia/reperfusion NF-κB PPARγ COX-2 IкBα |
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