| 齐 洁,张 钧,马智超,傅 建,李淑艳.NPR-A/cGMP信号通路与自发性高血压大鼠左心室肥厚的关系及运动干预的效果分析[J].中国康复医学杂志,2014,29(4):338~342 |
| NPR-A/cGMP信号通路与自发性高血压大鼠左心室肥厚的关系及运动干预的效果分析 点此下载全文 |
| 齐 洁 张 钧 马智超 傅 建 李淑艳 |
| 扬州大学动物科学与技术学院，225009 |
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| 摘要
目的：观察运动对自发性高血压大鼠心钠素（ANP）含量、利钠肽受体A(NPR-A）mRNA表达及环磷酸鸟苷（cGMP）含量的影响，探讨NPR-A/cGMP信号通路与自发性高血压大鼠左心室肥厚的关系及运动干预的影响，为高血压及其并发症的防治提供理论依据。
方法：选用雄性Wistar大鼠8只作为正常对照组(WC组)，同时选用雄性自发性高血压大鼠（SHR）16只，随机分为高血压对照组(SC组)和高血压运动组(SE组)，每组8只。SE组每日进行60min的无负重游泳运动，每周6次，持续8周。每周日采用无创伤血压测定仪测定大鼠的收缩压与舒张压，运动8周后取材并计算大鼠心脏重量指数（HWI）和左心室质量指数（LVI），分别采用放射免疫法测定大鼠血清和心肌组织ANP含量，实时荧光定量PCR检测大鼠心肌NPR-A mRNA表达，酶联免疫吸附法（ELISA）测定大鼠心肌组织cGMP含量。
结果：8周实验后，SC组大鼠血压较实验前显著升高(P<0.01)，SE组较SC组大鼠血压显著下降(P<0.01)，且与实验前相比差异无显著性（P>0.05）；SC组大鼠HWI 和LVI 均显著高于SE组大鼠（P<0.05）；SE组大鼠血浆和心肌组织ANP含量虽升高但差异无显著性（P>0.05）；SE组大鼠心肌NPR-A mRNA表达量和cGMP含量较SC组大鼠显著升高（P<0.01）。
结论：8周60min游泳运动可以升高SHR心肌NPR-A mRNA的表达水平，其与ANP结合后可增加SHR心肌第二信使cGMP的含量，从而发挥ANP在降压和抗心肌肥大中的作用。 |
| 关键词：高血压 心肌肥厚 运动 心房利钠肽 利钠肽受体A 环磷酸鸟苷 |
| Natriuretic peptide receptor-A/cyclic guanosine monophosphate signaling pathway and left ventricular hypertrophy of spontaneous hypertensive rats and the effect of exercise intervention Download Fulltext |
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| College of Animal Science and Technology, Yangzhou University, 225009 |
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| Abstract: |
| Abstract
Objective：To study the effect of exercise intervention on left ventricular hypertrophy in spontaneously hypertensive rats(SHR) by determining the mRNA expression of natriuretic peptide receptor A（NPRA） and the contents of atrial natriuretic peptide（ANP）, cyclic guanosine monophosphate（cGMP） in cardiac muscle.
Method：Sixteen male SHR were randomly divided into SHR control group(SC,n=8) and SHR exercise training group (SE,n=8), another eight male Wistar rats were as normal matched control group (WC). SE group carried on 60min swimming 6 times a week, all rats feed normal feedstuff for 8 weeks. And then the rats' blood pressure were determined weekly, the contents of ANP were detected by radioimmunoassay, cGMP in cardiac muscle by ELISA, and the mRNA expression of NPR in cardiac muscle by real time-PCR.
Result：After 8-week exercise, BP of SE group was little higher than before, but no significant difference. However, that of SC group was just opposite (P<0.01); To compared with SC group, in SE group BP, index of HWI and LVI decreased dramatically(P<0.01), the level of ANP increased, but no significant difference; the level of NPR mRNA expression and cGMP content elevated markedly(P<0.01).
Conclusion: The 8-week 60-min swimming exercise can increase the ANP amount of plasma in SHR. Meanwhile, the expression of NPR-A mRNA (the ANP receptor) and content of cGMP in cardiac muscle also increased, then ANP play a role against myocardial hypertrophy and proliferation. |
| Keywords:hypertension cardiac hypertrophy exercise atrial natriuretic peptide natriuretic peptide receptor-A cyclic guanosine monophosphate |
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