| 张 璇,张林亭,张 霞.强制性使用运动疗法对大鼠脑缺血后神经可塑性的影响[J].中国康复医学杂志,2014,29(7):615~618 |
| 强制性使用运动疗法对大鼠脑缺血后神经可塑性的影响 点此下载全文 |
| 张 璇 张林亭 张 霞 |
| 济宁市第一人民医院神经科，272000 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 1837 |
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| 摘要： |
| 摘要
目的：观察强制性使用运动疗法对大鼠脑梗死区周围皮质的生长相关蛋白-43(GAP-43)、突触素（SYP）表达的影响，探讨其促进脑缺血后神经可塑性的机制。
方法：采用自体血栓注入并闭塞大脑中动脉的方法制备局灶脑缺血模型。 将90只健康SD大鼠随机分为假手术组(Sham组)、局灶脑缺血模型组（MCAO模型）、强制性运动疗法组( CIMT 组)各 30只。在局灶脑缺血模型成功后7天，运动疗法组建立强制性使用运动疗法模型。参照Bederson评分标准对各组大鼠进行神经功能缺损评分。采用原位杂交技术检测脑梗死区周围皮质GAP-43及SYP mRNA的表达。采用免疫组化技术检测GAP-43及SYP蛋白表达。
结果：与模型组比较，运动疗法组在制模后2、4、8周神经功能缺损评分明显降低，而GAP-43及突触素mRNA及蛋白表达在各时间点均明显高于对照组。
结论：强制性使用运动疗法能改善大鼠脑缺血神经功能，其机制可能与运动疗法能够促进梗死灶周围皮质GAP-43及SYP的表达，从而促进脑缺血后的突触发生与重塑有关。 |
| 关键词：强制性使用运动疗法 脑缺血 生长相关蛋白 突触素 |
| Effects of constraint-induced-movement therapy on neural plasticity after cerebral ischemia in rats Download Fulltext |
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| The Department of Neurology,The First People's Hospital of Jining, Shandong,272000 |
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| Abstract: |
| Abstract
Objective:To investigate the effects of constraint-induced movement therapy (CIMT) on the expressions of growth-associated protein 43(GAP-43) and synaptophysin(SYP) after focal cerebral ischemia in rats．
Method:A focal cerebral ischemia model of rat was established by embolizing left middle cerebral artery with autologous blood clots．Ninety healthy Sprague-Dawley rats were randomly divided into 3 groups: sham group，CIMT group，and focal cerebral ischemia（MCAO） group，30 in each group. The CIMT group rats were forced to use their impaired limbs by placing their nonimpaired fore-limbs in casts at the 7th day after MCAO. The behavior scores were evaluated. Expressions of GAP-43, SYPmRNA and protein were detected by means of in situ hybridization histochemistry technique and immunohistochemistry.
Result:Compared with MCAO group, the scores of neurological deficit were significantly lower， and the expressions of GAP-43, SYPmRNA and protein were obviously higher in CIMT group.
Conclusion:CIMT could improve neurological function of rats after cerebral ischemia.The possible mechanism was closely related to up-regulation of expressions of GAP-43 and SYP, thus to improve neural plasticity. |
| Keywords:constraint-induced movement therapy cerebral ischemia growth-associated protein-43 synaptophysin |
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